Wood J M, Schild G C, Newman R W
J Gen Virol. 1980 Apr;47(2):355-63. doi: 10.1099/0022-1317-47-2-355.
A novel radial-diffusion technique in agarose gels is described which is applicable to the assay of small concentrations of influenza haemagglutinin (HA) antigen. The method is based on single-radial diffusion zone size enhancement (ZE) using autoradiography to demonstrate antigen--antibody reactions. The ZE technique is capable of reliably assaying concentrations of haemagglutinin as low as 0.1 microgram/ml, which represents a 20- to 40-fold increase in sensitivity over conventional single-radial-diffusion techniques. The ZE response is dependent upon the antigenic identity of the 125I-labelled 'marker' HA and the HA antigen being assayed. Haemagglutinins showing variation within a subtype (e.g. H3) show reduced ZE responses and thus the method has potential value for distinguishing between closely related HA antigens.
本文描述了一种适用于检测低浓度流感血凝素（HA）抗原的新型琼脂糖凝胶径向扩散技术。该方法基于使用放射自显影来证明抗原 - 抗体反应的单径向扩散区大小增强（ZE）技术。ZE技术能够可靠地检测低至0.1微克/毫升的血凝素浓度，这比传统的单径向扩散技术灵敏度提高了20至40倍。ZE反应取决于125I标记的“标记”HA和所检测的HA抗原的抗原同一性。在一个亚型（例如H3）内表现出变异的血凝素显示出降低的ZE反应，因此该方法对于区分密切相关的HA抗原具有潜在价值。
