Rozental S, Alviano C S, de Souza W
Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brasil.
Mycopathologia. 1994 May;126(2):85-91. doi: 10.1007/BF01146200.
Light and electron microscopy were used to analyze in vitro the interaction of Fonsecaea pedrosoi with in vivo activated-macrophages. Adherence of the fungi to the surface of activated macrophages triggers the respiratory burst as revealed by reduction of nitroblue tetrazolium. Transmission electron microscopy revealed NAD(PO)H-oxidase activity in the portions of the macrophage plasma of membrane that were in contact with the fungus as well as within phagocytic vacuoles. Activated macrophages failed to kill ingested fungi, but they showed a fungistatic activity delaying germ tube and hyphae formation.
利用光学显微镜和电子显微镜在体外分析裴氏着色真菌与体内活化巨噬细胞的相互作用。真菌附着于活化巨噬细胞表面会引发呼吸爆发，这可通过硝基蓝四氮唑的还原得以揭示。透射电子显微镜显示，在巨噬细胞质膜与真菌接触的部分以及吞噬泡内存在NAD(PO)H氧化酶活性。活化巨噬细胞无法杀死摄入的真菌，但它们表现出抑菌活性，可延迟芽管和菌丝的形成。
