Blecher M, Lewis S, Hicks J M, Josephs S
J Allergy Clin Immunol. 1984 Sep;74(3 Pt 1):246-51. doi: 10.1016/0091-6749(84)90253-7.
We have used the inhibition of binding of a potent beta antagonist, iodohydroxybenzylpindolol, to canine lung-plasma membrane beta-adrenergic receptors as a test for beta-blocking autoantibodies in the sera of 376 mildly and severely asthmatic children. This binding inhibition assay, coupled with a variant test in which the binding assay was performed on selected sera (binding values below 2 SD from the mean) before and after immunodepletion of the sera (removal of IgG and IgA), permitted the conclusion that about 5% of the juvenile asthmatic population studied produce beta-blocking autoantibodies.
我们采用一种强效β拮抗剂——碘羟基苄基吲哚洛尔与犬肺细胞膜β-肾上腺素能受体结合的抑制作用，来检测376名轻、重度哮喘儿童血清中的β阻断自身抗体。这种结合抑制试验，再加上一种变体试验，即在对血清进行免疫去除（去除IgG和IgA）前后，对选定血清（结合值低于平均值2个标准差）进行结合试验，从而得出结论：在所研究的青少年哮喘患者群体中，约5%的人会产生β阻断自身抗体。
