Malet-Martino M C, Betbeder D, Lattes A, Lopez A, Martino R, Francois G, Cros S
J Pharm Pharmacol. 1984 Aug;36(8):556-9. doi: 10.1111/j.2042-7158.1984.tb04454.x.
Quantitative determination of the intravascular persistence of F-tri-n-butylamine (FC 43 as Fluosol 43) in mice was carried out using 19F nmr spectroscopy. The method allows the direct study of whole blood, neither separation nor extraction of the sample being required. Accuracy (reproducibility) is better than +/- 3%, and is comparable to that of the gas chromatographic (gc) method. The sensitivity of detection is less than that of the gc method but is sufficient for this biological study. It was observed that the intravascular elimination of F-tri-n-butylamine follows a non-linear kinetic and becomes faster about 40 h after the injection. This phenomenon may be explained by the size-increase of the FC 43 droplets in the emulsion. Indeed, at about 40 h after injection, the level of Pluronic F-68 in the bloodstream was no longer sufficient to maintain the stability of the FC-43 droplets. They therefore tended to coalesce forming larger droplets that were phagocytosed more rapidly by the histiocytes of the reticuloendothelial system.
利用19F核磁共振波谱法对小鼠体内F-三正丁胺（作为氟索43的FC 43）的血管内持久性进行了定量测定。该方法可直接对全血进行研究，无需对样品进行分离或提取。准确度（重现性）优于±3%，与气相色谱（gc）法相当。检测灵敏度低于gc法，但足以用于该生物学研究。观察到F-三正丁胺的血管内消除遵循非线性动力学，注射后约40小时消除速度加快。这种现象可能是由于乳剂中FC 43液滴尺寸增大所致。事实上，注射后约40小时，血流中普朗尼克F-68的水平已不足以维持FC-43液滴的稳定性。因此，它们倾向于聚结形成更大的液滴，这些液滴被网状内皮系统的组织细胞吞噬得更快。
