Savy C, Tamboise E
Radiat Environ Biophys. 1980 Feb;17(2):115-27. doi: 10.1007/BF02027848.
Pregnant rats were exposed to absorbed doses of 0.5, 1, 2, and 4 Gy of 60Co-gamma rays either on day 8 or on day 12 p.c.. The embryos were collected on day 18 p.c.. Irradiations both on day 8 and 12 p.c. result in a dose dependent decrement of weight. The effect is larger for irradiation on day 8 p.c.. Caryometric studies of the neurons in the trigeminal ganglion show, on the other hand, that the reduction of nuclear sizes in this tissue is more substantial for an irradiation on day 12 p.c. when the ganglion is in a stage of formation. Cytophotometric determinations of the Feulgen-DNA content and determination of the RNA content by staining and chromic gallocyanin in combination with ribinuclease digestion lead to the conclusion that the irradiation induces no significant hyperploidy and that the irradiated neurons have the nuclear RNA content that is normal at this stage of gestation. This applies both to the irradiations on day 8 and on day 12 p.c.
将怀孕大鼠在妊娠第8天或第12天接受0.5、1、2和4 Gy的60Co-γ射线吸收剂量照射。在妊娠第18天收集胚胎。妊娠第8天和第12天的照射均导致体重呈剂量依赖性下降。妊娠第8天照射的影响更大。另一方面，对三叉神经节神经元的细胞核测量研究表明，当神经节处于形成阶段时，妊娠第12天照射导致该组织细胞核大小的减小更为显著。通过Feulgen-DNA含量的细胞光度测定以及用铬性胆青素染色并结合核糖核酸酶消化来测定RNA含量，得出的结论是，照射不会诱导明显的超倍体现象，并且受照射的神经元在妊娠这个阶段具有正常的细胞核RNA含量。这适用于妊娠第8天和第12天的照射。
