Latif A A, Webster A D
J Immunol Methods. 1982;49(1):83-8. doi: 10.1016/0022-1759(82)90368-4.
A radioimmunoassay for type specific pneumococcal polysaccharides is described. This involves obtaining specific polysaccharides from culture supernatants by precipitation with ethanol saturated with potassium acetate, and then after further purification labelling the tyraminated polysaccharide with 125 iodine. The radioimmunoassay itself is a standard ammonium sulphate precipitation technique (Farr assay) using IgG myeloma protein as a carrier. We have found that measuring antibodies after test immunization with a polyvalent pneumococcal vaccine is a useful aid in diagnosing functional antibody deficiency.
本文描述了一种针对特定类型肺炎球菌多糖的放射免疫测定法。这包括通过用醋酸钾饱和的乙醇沉淀从培养上清液中获取特定多糖，然后在进一步纯化后用125碘标记经酪胺化的多糖。放射免疫测定本身是一种使用IgG骨髓瘤蛋白作为载体的标准硫酸铵沉淀技术（法尔氏测定法）。我们发现，在用多价肺炎球菌疫苗进行试验性免疫后测量抗体，有助于诊断功能性抗体缺乏。
