Chaussonnerie Sébastien, Saaidi Pierre-Loïc, Ugarte Edgardo, Barbance Agnès, Fossey Aurélie, Barbe Valérie, Gyapay Gabor, Brüls Thomas, Chevallier Marion, Couturat Loïc, Fouteau Stéphanie, Muselet Delphine, Pateau Emilie, Cohen Georges N, Fonknechten Nuria, Weissenbach Jean, Le Paslier Denis
Commissariat à l'Energie Atomique et aux Energies Alternatives, Direction de la Recherche Fondamentale, Institut de GénomiqueEvry, France; Université d'Evry Val d'EssonneEvry, France; Centre National de la Recherche Scientifique, UMR8030, Génomique métaboliqueEvry, France.
Commissariat à l'Energie Atomique et aux Energies Alternatives, Direction de la Recherche Fondamentale, Institut de Génomique Evry, France.
Front Microbiol. 2016 Dec 20;7:2025. doi: 10.3389/fmicb.2016.02025. eCollection 2016.
Chlordecone (Kepone®) is a synthetic organochlorine insecticide (CClO) used worldwide mostly during the 1970 and 1980s. Its intensive application in the French West Indies to control the banana black weevil led to a massive environmental pollution. Persistence of chlordecone in soils and water for numerous decades even centuries causes global public health and socio-economic concerns. In order to investigate the biodegradability of chlordecone, microbial enrichment cultures from soils contaminated by chlordecone or other organochlorines and from sludge of a wastewater treatment plant have been conducted. Different experimental procedures including original microcosms were carried out anaerobically over long periods of time. GC-MS monitoring resulted in the detection of chlorinated derivatives in several cultures, consistent with chlordecone biotransformation. More interestingly, disappearance of chlordecone (50 μg/mL) in two bacterial consortia was concomitant with the accumulation of a major metabolite of formula CClH (named B1) as well as two minor metabolites CClHO (named A1) and CClH (named B3). Finally, we report the isolation and the complete genomic sequences of two new isolates, closely related to , and that were capable of reproducing chlordecone transformation. Further characterization of these strains should yield deeper insights into the mechanisms involved in this transformation process.
开蓬（商品名：克蓬）是一种合成有机氯杀虫剂（CClO），在20世纪70年代和80年代广泛应用于世界各地。它在法属西印度群岛大量用于控制香蕉黑象甲，导致了大规模的环境污染。开蓬在土壤和水中持续存在数十年甚至数百年，引发了全球公共卫生和社会经济方面的担忧。为了研究开蓬的生物降解性，人们从受开蓬或其他有机氯污染的土壤以及污水处理厂的污泥中进行了微生物富集培养。包括原始微观世界在内的不同实验程序在厌氧条件下进行了很长时间。气相色谱 - 质谱监测结果显示在几种培养物中检测到了氯化衍生物，这与开蓬的生物转化一致。更有趣的是，在两个细菌群落中，开蓬（50μg/mL）的消失伴随着一种主要代谢产物CClH（命名为B1）以及两种次要代谢产物CClHO（命名为A1）和CClH（命名为B3）的积累。最后，我们报告了两个新分离菌株的分离和完整基因组序列，它们与[未提及的相关菌株]密切相关，并且能够重现开蓬的转化过程。对这些菌株的进一步表征应该能更深入地了解这一转化过程所涉及的机制。
