Zweig M, Heilman C J, Rabin H, Hampar B
J Virol. 1980 Sep;35(3):644-52. doi: 10.1128/JVI.35.3.644-652.1980.
Guinea pig antisera and mouse monoclonal antibodies against a 40,000-molecular-weight nucleocapsid protein (p40) of herpes simplex virus types 1 and 2 immunoprecipitated 40,000- and 80,000-molecular-weight classes of soluble proteins from infected cell extracts. The soluble 40,000-molecular-weight protein class (intracellular p40) appeared as a cluster of three to four closely spaced bands of proteins having molecular weights ranging between 39,000 and 45,000, whereas the soluble 80,000-molecular-weight protein class (intracellular p80) appeared as a doublet of bands. The peptide map of intracellular p40 closely resembled the maps of the p40 and p45 proteins of nucleocapsids, but it showed both differences and similarities when compared with the peptide map of intracellular p80. Pulse-chase experiments suggested that intracellular p80 was not a precursor of intracellular p40. We conclude that the intracellular p40 and p80 protein classes share common antigenic determinants, presumably reflecting similar amino acid sequences, although they have distinct differences in protein structure.
豚鼠抗血清和针对单纯疱疹病毒1型和2型40,000分子量核衣壳蛋白（p40）的小鼠单克隆抗体，从感染细胞提取物中免疫沉淀出40,000和80,000分子量的可溶性蛋白类别。可溶性40,000分子量蛋白类别（细胞内p40）表现为三到四条紧密间隔的蛋白条带簇，分子量在39,000至45,000之间，而可溶性80,000分子量蛋白类别（细胞内p80）表现为双条带。细胞内p40的肽图与核衣壳的p40和p45蛋白的图谱非常相似，但与细胞内p80的肽图相比，它显示出差异和相似之处。脉冲追踪实验表明，细胞内p80不是细胞内p40的前体。我们得出结论，细胞内p40和p80蛋白类别共享共同的抗原决定簇，大概反映了相似的氨基酸序列，尽管它们在蛋白质结构上有明显差异。
