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单纯疱疹病毒1型和2型细胞内p40：部分蛋白酶解产生的肽段上的型特异性和交叉反应性抗原决定簇

Herpes simplex virus type 1 and 2 intracellular p40: type-specific and cross-reactive antigenic determinants on peptides generated by partial proteolysis.

作者信息

Heilman C J, Zweig M, Hampar B

出版信息

J Virol. 1981 Nov;40(2):508-15. doi: 10.1128/JVI.40.2.508-515.1981.

DOI:10.1128/JVI.40.2.508-515.1981
PMID:6172597
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC256653/
Abstract

Intracellular p40 is a class of protein ranging in molecular weight from 39,000 to 45,000 that is immunoprecipitated from herpes simplex virus type 1 (HSV-1)- and HSV-2-infected cell extracts by mouse monoclonal antibodies or guinea pig antisera against HSV-1 and HSV-2 nucleocapsid p40. Analysis by a two-dimensional gel system showed that HSV-1 and HSV-2 intracellular p40 each consisted of three major components. However, these HSV-1 and HSV-2 proteins differed in charge and size. Analysis of Staphylococcus aureus V8 protease partial digests by two-dimensional gel electrophoresis indicated that none of the peptides of HSV-1 and HSV-2 intracellular p40 were identical. Immunoprecipitation of the partial digest products of intracellular p40-1 and p40-2 with homologous and heterologous guinea pig antisera resulted in the precipitation of various combinations of peptides indicating the presence of either type-specific or cross-reactive antigenic determinants.

摘要

细胞内p40是一类分子量在39,000至45,000之间的蛋白质，可通过小鼠单克隆抗体或针对单纯疱疹病毒1型（HSV-1）和HSV-2核衣壳p40的豚鼠抗血清，从感染HSV-1和HSV-2的细胞提取物中免疫沉淀得到。二维凝胶系统分析表明，HSV-1和HSV-2的细胞内p40各自均由三个主要成分组成。然而，这些HSV-1和HSV-2蛋白在电荷和大小上有所不同。通过二维凝胶电泳对金黄色葡萄球菌V8蛋白酶部分消化产物的分析表明，HSV-1和HSV-2细胞内p40的肽段均不相同。用同源和异源豚鼠抗血清对细胞内p40-1和p40-2的部分消化产物进行免疫沉淀，导致各种肽段组合的沉淀，表明存在型特异性或交叉反应性抗原决定簇。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/472f/256653/dd0600ab16f4/jvirol00164-0189-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/472f/256653/2e7c03576213/jvirol00164-0186-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/472f/256653/a77962f9a082/jvirol00164-0187-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/472f/256653/603fccc9f2c0/jvirol00164-0188-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/472f/256653/78e720dec04e/jvirol00164-0188-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/472f/256653/3a2f4a2e94e4/jvirol00164-0189-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/472f/256653/dd0600ab16f4/jvirol00164-0189-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/472f/256653/2e7c03576213/jvirol00164-0186-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/472f/256653/a77962f9a082/jvirol00164-0187-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/472f/256653/603fccc9f2c0/jvirol00164-0188-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/472f/256653/78e720dec04e/jvirol00164-0188-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/472f/256653/3a2f4a2e94e4/jvirol00164-0189-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/472f/256653/dd0600ab16f4/jvirol00164-0189-b.jpg

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本文引用的文献

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Infect Immun. 1980 Aug;29(2):724-32. doi: 10.1128/iai.29.2.724-732.1980.
2
Shared antigenic determinants between two distinct classes of proteins in cells infected with herpes simplex virus.感染单纯疱疹病毒的细胞中两类不同蛋白质之间的共同抗原决定簇。
J Virol. 1980 Sep;35(3):644-52. doi: 10.1128/JVI.35.3.644-652.1980.
3
Proteins specified by herpes simplex virus. XI. Identification and relative molar rates of synthesis of structural and nonstructural herpes virus polypeptides in the infected cell.
将单纯疱疹病毒感染细胞的变性、经电泳分离并固定的裂解物用于单克隆抗体检测及病毒蛋白特性研究。
J Virol. 1983 Apr;46(1):103-12. doi: 10.1128/JVI.46.1.103-112.1983.
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Localization of a type-specific antigenic site on herpes simplex virus type 2 glycoprotein D.2型单纯疱疹病毒糖蛋白D上一种型特异性抗原位点的定位
J Virol. 1984 Jul;51(1):263-5. doi: 10.1128/JVI.51.1.263-265.1984.
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J Virol. 1983 Jul;47(1):185-92. doi: 10.1128/JVI.47.1.185-192.1983.
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Infect Immun. 1985 Jun;48(3):806-12. doi: 10.1128/iai.48.3.806-812.1985.
单纯疱疹病毒指定的蛋白质。十一。感染细胞中单纯疱疹病毒结构和非结构多肽的合成鉴定及相对摩尔率。
J Virol. 1973 Dec;12(6):1347-65. doi: 10.1128/JVI.12.6.1347-1365.1973.
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J Gen Virol. 1973 May;19(2):217-33. doi: 10.1099/0022-1317-19-2-217.
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J Biol Chem. 1977 Feb 10;252(3):1102-6.
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