Sloan T B, Martincic R R, Anderson B
Exp Cell Biol. 1981;49(1):20-33. doi: 10.1159/000163775.
Antisera to cultured human synovial cells were produced in several rabbits by a variety of immunization procedures. The antisera were assayed by a number of immunologic techniques for specificities against live synovial and other fibroblastic cell lines and against additional antigen sources including human serum and synovial fluid, and fetal calf serum. While the antisera reacted well and with the same titers to all synovial cell lines, reactivities varied with several nonsynovial cell lines showing that synovial tissue-derived cells are antigenically different from other tissue fibroblastic cell strains. Also, the major antigens cross-reactive between synovial and certain other fibroblastic cell lines were partially defined as those in or cross-reactive with fetal calf serum and human serum or plasma components. The differential reactivities of the antisera demonstrated that the antibody specificity populations were markedly affected by the immunization manipulations. Methods for analyzing those differences are presented by defining specificity and reactivity indices. The antisera to the synovial cells distinguished 7 electrophoretically distinct reactivities in human serum or plasma, synovial fluid, and in fetal calf serum. 1 antigen was identified as fibronectin, 3 as components of fetal calf serum and 3 additional unidentified human serum cross-reactive antigens.
通过多种免疫程序在几只兔子体内制备了针对培养的人滑膜细胞的抗血清。采用多种免疫技术对抗血清进行检测，以确定其对活的滑膜和成纤维细胞系以及包括人血清、滑液和胎牛血清在内的其他抗原来源的特异性。虽然抗血清对所有滑膜细胞系反应良好且效价相同，但对几种非滑膜细胞系的反应性有所不同，这表明滑膜组织来源的细胞在抗原性上与其他组织的成纤维细胞系不同。此外，滑膜与某些其他成纤维细胞系之间交叉反应的主要抗原部分被确定为胎牛血清、人血清或血浆成分中的抗原或与之交叉反应的抗原。抗血清的不同反应性表明抗体特异性群体受到免疫操作的显著影响。通过定义特异性和反应性指数，提出了分析这些差异的方法。滑膜细胞抗血清在人血清或血浆、滑液和胎牛血清中区分出7种电泳上不同的反应性。1种抗原被鉴定为纤连蛋白，3种为胎牛血清成分，还有3种未鉴定的人血清交叉反应抗原。
