Torack R M
Histochemistry. 1980;68(3):297-307. doi: 10.1007/BF00493258.
Ultrastructural studies of the subependymal neuropil of the lateral ventricular wall in the adult rat reveal two different types of enlarged extracellular spaces which appear to be due to the presence of glycosaminoglycans. Focal irregular enlargements are more numerous and are distinguished by the presence of fibrillar material. The other network is characterized by uniform width, branching processes, and an electron density identical to vascular basement membranes. The origin of the extracellular material is not revealed by these studies, but a relationship is suggested between the focal dilatation and subependymal cells. The distinction of these spaces is emphasized by the study of neonatal brain and by the use of ruthenium red (RR). Focal dilatations are very prominent in the 10 day old rat, but they do not contain fibrillar material. An adult type basement membrane-like network is present at this time. In the adult rat the focal dilatation has great affinity for RR, but the second network is very sparsely stained. The RR affinity is not present in the 10 day old rat; however, by the 26th day, the affinity is almost as great as that of an adult. These findings are considered to enhance the concept of a separate origin, composition, and probable function of these two networks.
成年大鼠侧脑室壁室管膜下神经纤维网的超微结构研究显示，存在两种不同类型的扩大细胞外间隙，这似乎是由于糖胺聚糖的存在所致。局灶性不规则扩大更为常见，其特征是存在纤维状物质。另一种网络的特征是宽度均匀、有分支且电子密度与血管基底膜相同。这些研究未揭示细胞外物质的来源，但提示局灶性扩张与室管膜下细胞之间存在关联。对新生脑的研究以及使用钌红（RR）强调了这些间隙的差异。局灶性扩张在10日龄大鼠中非常明显，但其中不含纤维状物质。此时存在成年型基底膜样网络。在成年大鼠中，局灶性扩张对RR有很强的亲和力，但第二种网络染色非常稀疏。10日龄大鼠中不存在RR亲和力；然而，到第26天时，亲和力几乎与成年大鼠一样强。这些发现被认为强化了这两种网络具有不同起源、组成和可能功能的概念。
