Lett-Brown M A, Aelvoet M, Hooks J J, Georgiades J A, Thueson D O, Grant J A
J Clin Invest. 1981 Feb;67(2):547-52. doi: 10.1172/JCI110065.
It is well established that viral infections may precipitate or worsen attacks of bronchial asthma. Furthermore, in symptomatic atopic subjects, the local accumulation of basophils and the production of a basophil chemotactic factor have been reported. We have investigated the effect of cell-free supernates from viral stimulated cultures of human mononuclear cells on the in vitro migration of human basophils. Our results show the presence of a factor in these culture supernates that enhances the migration of basophils toward two separate chemoattractants, a peptide from C5 and a lymphokine. The enhancing activity, while affecting basophil migration, did not change the response of monocytes. The enhancing activity resembled viral-induced interferon when (a) pH 2 stability, (b) heat resistance, (c) trypsin sensitivity, and (d) species-specificity were compared. Finally, the enhancing activity for basophil chemotaxis and the interferon titer were highly correlated in preparations with a 10(4)-fold difference in interferon specific activity. Our studies show that viral-induced interferon can augment the in vitro chemotactic response of basophils. Because mediators present in basophils may be involved in the pathogenesis of immediate hypersensitivity, the modulation of basophil movement by interferon suggests a possible mechanism for the association between viral infections and atopic disorders.
病毒感染可能会引发或加重支气管哮喘发作，这一点已得到充分证实。此外，据报道，在有症状的特应性受试者中，嗜碱性粒细胞会在局部积聚并产生嗜碱性粒细胞趋化因子。我们研究了人单核细胞病毒刺激培养物的无细胞上清液对人嗜碱性粒细胞体外迁移的影响。我们的结果表明，这些培养上清液中存在一种因子，可增强嗜碱性粒细胞向两种不同趋化因子（C5衍生肽和一种淋巴因子）的迁移。这种增强活性在影响嗜碱性粒细胞迁移时，并未改变单核细胞的反应。当比较（a）pH 2稳定性、（b）耐热性、（c）胰蛋白酶敏感性和（d）种属特异性时，这种增强活性类似于病毒诱导的干扰素。最后，在干扰素比活性相差10⁴倍的制剂中，嗜碱性粒细胞趋化增强活性与干扰素滴度高度相关。我们的研究表明，病毒诱导的干扰素可增强嗜碱性粒细胞的体外趋化反应。由于嗜碱性粒细胞中存在的介质可能参与速发型超敏反应的发病机制，干扰素对嗜碱性粒细胞运动的调节提示了病毒感染与特应性疾病之间关联的一种可能机制。
