Koch G, Osmond D G, Julius M H, Benner R
J Immunol. 1981 Apr;126(4):1447-51.
During the primary immune response of mice to i.v. administered thymus-dependent antigens the spleen is the major site of localization of antibody-producing plaque-forming cells (PFC). During the secondary response, on the other hand, large numbers of PFC not only appear in the spleen, but also in the bone marrow. By inducing B memory cells with a DNP-carrier complex and activating the DNP-specific B memory cells with the same hapten conjugated to a heterologous carrier, we show in this paper that B memory cells, but not necessarily T memory cells, must be present before booster immunization for PFC to appear in the bone marrow. The origin of the PFC that appear in the bone marrow during secondary type immune response was studied in parabiotic mice consisting of members congenic for the Igh-1 locus. From analysis of the allotype of antibodies produced by PFC in the marrow of such pairs of parabionts it appeared that antibody formation in bone marrow is dependent on the immigration into the marrow of B memory cells activated in peripheral lymphoid organs. Consistent with such a migration of activated cells, radioautographic studies in guinea pigs demonstrated an influx of newly formed mononuclear cells into the bone marrow via the blood stream during the first 3 days after intravascular antigen administration.
在小鼠对静脉注射胸腺依赖性抗原的初次免疫应答过程中，脾脏是产生抗体的斑块形成细胞（PFC）定位的主要部位。另一方面，在二次应答期间，大量PFC不仅出现在脾脏中，也出现在骨髓中。通过用二硝基苯（DNP）-载体复合物诱导B记忆细胞，并用与异源载体偶联的相同半抗原激活DNP特异性B记忆细胞，我们在本文中表明，在加强免疫之前必须存在B记忆细胞，但不一定是T记忆细胞，以便PFC出现在骨髓中。在由Igh-1位点同基因的成员组成的联体小鼠中，研究了二次免疫应答期间出现在骨髓中的PFC的来源。通过分析此类联体小鼠骨髓中PFC产生的抗体的同种异型，发现骨髓中的抗体形成依赖于在外周淋巴器官中激活的B记忆细胞迁移到骨髓中。与活化细胞的这种迁移一致，豚鼠的放射自显影研究表明，在血管内注射抗原后的头3天内，新形成的单核细胞通过血流流入骨髓。
