Mehta P D, Patrick B A, Thormar H
Immunology. 1982 Jun;46(2):423-8.
Sera and cerebrospinal fluid (CSF) from five subacute sclerosing panencephalitis (SSPE) patients were absorbed with a concentrated preparation of cell cultures infected with measles virus. The precipitates containing measles antigen-antibody complex were washed and measles-specific IgG was eluted at low pH. When the profiles of measles-specific IgG eluted from sera and the corresponding CSF were compared in immunofixation after isoelectric focusing they showed almost identical oligoclonal band patterns with respect to number, intensity, isoelectric point and light chain class. The results suggest that the same cell clones are responsible for the synthesis of measles-specific IgG in the central nervous system and serum of patients with SSPE.
从5例亚急性硬化性全脑炎（SSPE）患者采集的血清和脑脊液（CSF），用感染麻疹病毒的细胞培养浓缩制剂进行吸附处理。含有麻疹抗原-抗体复合物的沉淀物经过洗涤，在低pH值条件下洗脱麻疹特异性IgG。在等电聚焦后的免疫固定分析中，比较从血清和相应脑脊液中洗脱的麻疹特异性IgG图谱时，发现它们在条带数量、强度、等电点和轻链类别方面呈现出几乎相同的寡克隆带型。结果表明，相同的细胞克隆负责SSPE患者中枢神经系统和血清中麻疹特异性IgG的合成。
