Schaffalitzky de Muckadell O B, Fahrenkrug J, Holst J J
Scand J Gastroenterol. 1977;12(3):267-72. doi: 10.3109/00365527709180927.
The concentration of immunoreactive secretin in arterial blood and the exocrine pancreatic secretion were measured during intraportal infusion of secretin in doses of 0.03, 0.06, 0.1 and 3.0 clinical units kg-1 h-1, and during intraduodenal instillation of 50 ml 0.1 mol 1(-1) HC1. The lowest dose of exogenous secretin to significantly increase secretin concentration in blood was 0.03 clinical units kg-1 h-1, which was a subthreshold dose of exocrin pancreatic secretion. A linear relation was found between the dose secretin and the secretin concentrations measured. On the basis of secretin concentrations, release of secretin during instillation of HC1 was estimated to be 0.22 clinical units kg-1 h-1. Maximum pancreatic bicarbonate secretion was obtained with a dose of 1.0 clinical units kg-1 h-1, and the minimal effective dose was between 0.06 and 0.1 clinical units kg-1 h-1. On the basis of the flow rate of pancreatic juice and the pancreatic bicarbonate output, secretin during instillation of HC1 was estimated to be 0.3 clinical unit kg-1 h-1. It is concluded that the radioimmunoassay used has the sensitivity and accuracy necessary for measurements of secretin concentrations in plasma during physiological conditions.
在以0.03、0.06、0.1和3.0临床单位·kg⁻¹·h⁻¹的剂量进行门静脉内注射促胰液素期间，以及在十二指肠内滴注50 ml 0.1 mol·l⁻¹盐酸期间，测量了动脉血中免疫反应性促胰液素的浓度和胰腺外分泌。能显著提高血液中促胰液素浓度的外源性促胰液素的最低剂量为0.03临床单位·kg⁻¹·h⁻¹，这是胰腺外分泌的阈下剂量。发现促胰液素剂量与所测促胰液素浓度之间呈线性关系。根据促胰液素浓度，估计在滴注盐酸期间促胰液素的释放量为0.22临床单位·kg⁻¹·h⁻¹。剂量为1.0临床单位·kg⁻¹·h⁻¹时获得最大胰腺碳酸氢盐分泌，最小有效剂量在0.06至0.1临床单位·kg⁻¹·h⁻¹之间。根据胰液流速和胰腺碳酸氢盐输出量，估计在滴注盐酸期间促胰液素为0.3临床单位·kg⁻¹·h⁻¹。结论是，所使用的放射免疫测定法具有在生理条件下测量血浆中促胰液素浓度所需的灵敏度和准确性。
