运用低角度激光光散射技术结合高压硅胶色谱法对米曲霉α淀粉酶分子量进行确证。
Confirmation of molecular weight of Aspergillus oryzae alpha-amylase using the low angle laser light scattering technique in combination with high pressure silica gel chromatography.
作者信息
Takagi T
出版信息
J Biochem. 1981 Feb;89(2):363-8. doi: 10.1093/oxfordjournals.jbchem.a133210.
Abstract
Molecular weight of Aspergillus oryzae alpha-amylase (Taka-amylase A) was estimated to be 51,000 +/- 500 by the combined use of high pressure silica gel (TSK-GEL G3000SW) chromatography and the low angle laser light scattering technique. The study was carried out partly to assess the performance of the combined technique, and results obtained indicate that it is highly promising as a method to determined protein molecular weight both accurately and quickly.
摘要
通过联用高压硅胶（TSK-GEL G3000SW）色谱法和低角度激光光散射技术，米曲霉α-淀粉酶（高峰淀粉酶A）的分子量估计为51,000±500。本研究部分是为了评估该联用技术的性能，所得结果表明，作为一种准确快速测定蛋白质分子量的方法，它极具前景。
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