转录依赖于recA功能的大肠杆菌突变体。
Escherichia coli mutants in which transcription is dependent on recA function.
作者信息
Froehlich B, Epstein W
出版信息
J Bacteriol. 1981 Sep;147(3):1117-20. doi: 10.1128/jb.147.3.1117-1120.1981.
Abstract
A gene essential for viability in recA mutants of Escherichia coli K-12 was identified. This gene, rdg for recA-dependent growth, is near 16 min on the E. coli chromosome between phr and gltA and is 90% cotransduced with gltA. In a strain with an rdg deletion and the temperature-sensitive recA allele, recA200, growth stopped within 7 min after cells were shifted to the nonpermissive temperature (42 degrees C). The cells remained viable for many hours at 42 degrees C. The defect at the nonpermissive temperature is in ribonucleic acid synthesis, which was completely shut off within 20 min after the temperature shift. Protein synthesis was also shut off, but deoxyribonucleic acid synthesis continued for at least 2 h after the shift. The rdg mutation alone had no apparent effect on growth, deoxyribonucleic acid repair, or recombination.
摘要
在大肠杆菌K-12的recA突变体中鉴定出一个对生存能力至关重要的基因。这个基因，即recA依赖性生长的rdg基因，位于大肠杆菌染色体上phr和gltA之间约16分钟处，与gltA的共转导率为90%。在一个具有rdg缺失和温度敏感型recA等位基因recA200的菌株中，细胞转移到非允许温度（42℃）后7分钟内生长停止。细胞在42℃下仍能存活数小时。在非允许温度下的缺陷在于核糖核酸合成，温度转移后20分钟内完全停止。蛋白质合成也停止了，但脱氧核糖核酸合成在转移后至少持续2小时。单独的rdg突变对生长、脱氧核糖核酸修复或重组没有明显影响。
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