Springer G F, Tegtmeyer H
Immunol Commun. 1981;10(2):157-71. doi: 10.3109/08820138109050694.
Terminal beta-D-galactopyranosyl (Gal) groups are implied in blood group N but not M specificity by the following findings: (a) Rabbit anti-asialoganglioside sera specific for terminal beta-Gal-(1 leads to 3)-GalNac agglutinate human group 0 M and N erythrocytes, the latter to a significantly higher titer, while rabbit anti-ganglioside GMl sera, whereas sialic acid modifies the antibody specificity, do not. The agglutination score with N erythrocytes was about twice that with M red blood cells. The asialoganglioside antibodies were readily absorbed by group 0 N erythrocytes, which were up to 10 times more efficient than group 0 M erythrocytes. Erythrocyte agglutination by the anti-asialoganglioside sera was inhibited by M and N antigen preparations isolated from group 0 red cells ghosts. N antigen was a better inhibitor. Asialoganglioside effectively inhibited the red cell agglutinations by anti-asialoganglioside serum. Ganglioside GMl did not inhibit. (b) Horse anti-pneumococcus Type XIV serum, which has anti-beta-Gal specificity, precipitated highly active N but not M substances. This precipitation was specifically inhibitable by oligosaccharides with terminal beta-Gal. (c) Beta galactosidase specifically inactivated native N and "acid-produced' N substances with the release of about three moles Gal per subunit of N antigen. It did not affect M antigen.
以下研究结果表明，末端β-D-吡喃半乳糖基（Gal）基团与血型N特异性有关，而与M特异性无关：（a）对末端β-Gal-（1→3）-GalNac具有特异性的兔抗去唾液酸神经节苷脂血清能凝集人0型M和N红细胞，其中对N红细胞的凝集效价显著更高，而兔抗神经节苷脂GM1血清（尽管唾液酸会改变抗体特异性）则不能。N红细胞的凝集评分约为M红细胞的两倍。去唾液酸神经节苷脂抗体很容易被0型N红细胞吸收，其吸收效率比0型M红细胞高10倍。抗去唾液酸神经节苷脂血清引起的红细胞凝集可被从0型红细胞膜中分离出的M和N抗原制剂所抑制。N抗原是更好的抑制剂。去唾液酸神经节苷脂能有效抑制抗去唾液酸神经节苷脂血清引起的红细胞凝集。神经节苷脂GM1则不能抑制。（b）具有抗β-Gal特异性的马抗肺炎双球菌 XIV 型血清能沉淀高活性的N物质，但不能沉淀M物质。这种沉淀可被具有末端β-Gal的寡糖特异性抑制。（c）β-半乳糖苷酶能特异性地使天然N物质和“酸产生的”N物质失活，每亚基N抗原释放约三摩尔Gal。它不影响M抗原。
