Drzeniek Z, Kúsnierz G, Lisowska E
Immunol Commun. 1981;10(2):185-97. doi: 10.3109/08820138109050696.
In the serum of a healthy blood donor, Mar, antibodies were identified that agglutinate blood group M erythrocytes only after their preincubation in glucose-containing solution. This agglutination was inhibited by blood group M glycoprotein preincubated in glucose solution but not by untreated M and N glycoproteins or N glycoprotein pretreated with glucose. A similar "activating' effect on M erythrocytes or M glycoprotein was achieved when glucose was replaced by mannose or N-acetylglucosamine, whereas several other replaced by mannose or N-acetylglucosamine, whereas several other sugars were ineffective. The "activating' effect of glucose on M antigen was time-, temperature-, pH-, and concentration-dependent. The binding of glucose to M glycoprotein was demonstrated under the preincubation conditions used in the above mentioned agglutinations. The results obtained suggest that the antibodies of serum, Mar, react with blood group M determinants modified by formation of a glycosylamine linkage between amino groups of the glycoprotein and glucose or other hexoses with the same configuration at asymmetric carbons 3, 4 and 5 as glucose.
在一名健康献血者的血清中，发现了马尔抗体，该抗体仅在血型M红细胞于含葡萄糖溶液中预孵育后才会使其发生凝集。这种凝集反应可被在葡萄糖溶液中预孵育的血型M糖蛋白抑制，但未被未处理的M和N糖蛋白或经葡萄糖预处理的N糖蛋白抑制。当葡萄糖被甘露糖或N-乙酰葡糖胺取代时，对M红细胞或M糖蛋白可产生类似的“激活”作用，而其他几种糖则无此作用。葡萄糖对M抗原的“激活”作用具有时间、温度、pH和浓度依赖性。在上述凝集反应所用的预孵育条件下，证实了葡萄糖与M糖蛋白的结合。所得结果表明，血清马尔抗体与血型M决定簇发生反应，该决定簇是通过糖蛋白氨基与葡萄糖或其他在不对称碳原子3、4和5处具有与葡萄糖相同构型的己糖形成糖胺键而修饰的。
