Tucker P W, Marcu K B, Newell N, Richards J, Blattner F R
Science. 1979 Dec 14;206(4424):1303-6. doi: 10.1126/science.117549.
The complete nucleotide sequence of the gamma 2b constant region gene cloned from BALB/c liver DNA is reported. The sequence of approximately 1870 base pairs includes the 5' flanking, 3' untranslated, and 3' flanking regions and three introns. The C gamma 2b coding region is divided by these introns into four segments corresponding to the homology domains and hinge region of the protein. The introns separating the hinge from the CH2 domain and the CH2 from the CH3 domain are small (106 and 119 base pairs). A larger intervening sequence of 314 base pairs separates the CH1 and hinge regions. The stretch of DNA comprising this large intron plus the hinge shows a strong homology with the other CH domains.
报道了从BALB/c肝脏DNA中克隆的γ2b恒定区基因的完整核苷酸序列。约1870个碱基对的序列包括5'侧翼区、3'非翻译区和3'侧翼区以及三个内含子。Cγ2b编码区被这些内含子分成四个片段，分别对应于蛋白质的同源结构域和铰链区。将铰链区与CH2结构域以及CH2与CH3结构域分隔开的内含子较小（分别为106和119个碱基对）。一个314个碱基对的较大间隔序列将CH1和铰链区分隔开。包含这个大内含子加上铰链区的DNA片段与其他CH结构域显示出很强的同源性。
