Eisenberg A D, Marquis R E
Antimicrob Agents Chemother. 1981 May;19(5):807-12. doi: 10.1128/AAC.19.5.807.
The uptake of protons by intact cells of Streptococcus mutans GS-5 was measured directly by assessing the pH increase in lightly buffered cell suspensions as protons moved across the plasma membrane after acid pulses. The barrier function of the cell membrane for protons was disrupted completely by 5% (vol/vol) butanol. This function was compromised severely by the ionophores valinomycin and gramicidin and by the uncoupler carbonylcyanide-m-chlorophenyl hydrazone. Proton conduction across the membrane was also enhanced by fluoride. It appeared that HF traversed the membrane and acted as a carrier. Once in the relatively alkaline cytoplasm, HF dissociated to yield F- and H+, which acted to acidify the cytoplasm and reduced the pH difference across the membrane.
通过评估酸脉冲后质子穿过质膜时轻度缓冲的细胞悬液中的pH升高，直接测量变形链球菌GS-5完整细胞对质子的摄取。5%（体积/体积）丁醇可完全破坏细胞膜对质子的屏障功能。缬氨霉素、短杆菌肽等离子载体以及解偶联剂羰基氰化物间氯苯腙会严重损害该功能。氟化物也可增强质子跨膜传导。似乎HF穿过膜并充当载体。一旦进入相对碱性的细胞质中，HF就会解离产生F-和H+，它们会使细胞质酸化并降低跨膜的pH差异。
