肺炎克雷伯菌核糖体制剂中荚膜多糖的免疫保护活性不涉及核糖核酸。
Immunoprotective activity of capsular polysaccharide in Klebsiella pneumoniae ribosomal preparations does not involve ribonucleic acid.
作者信息
Riottot M M, Fournier J M
出版信息
Infect Immun. 1981 Oct;34(1):126-30. doi: 10.1128/iai.34.1.126-130.1981.
Abstract
Two peaks were obtained by cesium chloride density gradient ultracentrifugation of Klebsiella pneumoniae ribosomal preparations. Peak I contained capsular polysaccharide, lipopolysaccharide, protein, and less than 0.5% ribonucleic acid. Peak II consisted mainly of ribonucleic acid, with low amounts of protein and capsular polysaccharide. Expressed as capsular polysaccharide content, the 50% protective dose of peak I and of nonfractionated ribosomal preparations was nearly constant (2.6 and 1.2 ng, respectively). Since peak I contained less than 0.5% ribonucleic acid, these results provide evidence that ribosomal ribonucleic acid is not required for protection of mice by K. pneumoniae capsular polysaccharide which contaminates ribosomal preparations.
摘要
通过对肺炎克雷伯菌核糖体制剂进行氯化铯密度梯度超速离心获得了两个峰。峰I含有荚膜多糖、脂多糖、蛋白质以及少于0.5%的核糖核酸。峰II主要由核糖核酸组成，蛋白质和荚膜多糖含量较低。以荚膜多糖含量表示，峰I和未分级的核糖体制剂的50%保护剂量几乎恒定（分别为2.6和1.2纳克）。由于峰I含有少于0.5%的核糖核酸，这些结果提供了证据，表明污染核糖体制剂的肺炎克雷伯菌荚膜多糖对小鼠的保护作用并不需要核糖体核糖核酸。
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