Riottot M, Fournier J M, Pillot J
Infect Immun. 1979 May;24(2):476-82. doi: 10.1128/iai.24.2.476-482.1979.
Klebsiella pneumoniae ribosomal preparations protect mice immunized by the subcutaneous route against an intraperitoneal challenge of 100 50% lethal doses. The minimal protective doses are 5 and 0.4 micrograms of proteins for preparations extracted from strains of capsular serotypes 1 and 2, respectively. This difference in protective activity is also found in bacteria killed by Formalin. The protective activity of these preparations is not diminished by their purification on sucrose gradient, which eliminates most of the membrane vesicles which are visible by electron microscopy. The use of four strains of K. pneumoniae belonging to capsular serotypes 1 and 2 allowed us to show that the immunoprotective capacity of the ribosomal preparations was specific to the capsular serotype of the origin strain. This was confirmed by experiments in which the serum of immunized mice was transferred passively. The experimental data favor the presence in the ribosomal preparation of antigens belonging to the bacterial surface and resisting elimination by ultracentrifugation on sucrose gradient. Those surface antigens (possibly capsular polysaccharide) at least play a role in the orientation of the specificity of the protection induced by the ribosomal preparations.
肺炎克雷伯菌核糖体制剂可保护经皮下途径免疫的小鼠，使其免受100个50%致死剂量的腹腔攻击。对于从荚膜血清型1和2菌株中提取的制剂，最小保护剂量分别为5微克和0.4微克蛋白质。在经福尔马林杀死的细菌中也发现了这种保护活性的差异。这些制剂在蔗糖梯度上纯化后其保护活性并未降低，蔗糖梯度纯化可去除大部分在电子显微镜下可见的膜泡。使用四株属于荚膜血清型1和2的肺炎克雷伯菌，我们发现核糖体制剂的免疫保护能力对原始菌株的荚膜血清型具有特异性。通过被动转移免疫小鼠血清的实验证实了这一点。实验数据支持核糖体制剂中存在属于细菌表面且能抵抗蔗糖梯度超速离心去除的抗原。那些表面抗原（可能是荚膜多糖）至少在核糖体制剂诱导的保护特异性定向中发挥作用。
