Stiegler P, Carbon P, Zuker M, Ebel J P, Ehresmann C
C R Seances Acad Sci D. 1980 Dec 8;291(12):937-40.
We present a model for the secondary structure of 16S ribosomal RNA from E. coli. This model has been deduced by restricting the total number of theoretical base pairings using the following criteria: (1) susceptibility of residues towards enzymatic probes that are specific for either paired or single stranded regions; (2) reactivity of certain residues to chemical modification; (3) evidence for medium and long range interactions; (4) comparative analysis of ribosomal RNA sequences from other organisms.
我们提出了一个大肠杆菌16S核糖体RNA二级结构的模型。该模型是通过使用以下标准限制理论碱基配对总数推导出来的:(1)残基对双链或单链区域特异性酶探针的敏感性;(2)某些残基对化学修饰的反应性;(3)中远距离相互作用的证据;(4)其他生物体核糖体RNA序列的比较分析。