Akagi T, Sonobe H, Miyoshi I, Yoshimoto S
Acta Med Okayama. 1982 Oct;36(5):325-30. doi: 10.18926/AMO/30690.
Antiserum was produced in white rabbit by intravenously injecting living cells of a B cell acute lymphoblastic leukemia (ALL) line (BALL-1). The reactivity of the antiserum against various lymphoid cell lines was examined by membrane immunofluorescence after appropriate absorption. Serum absorbed with non-T, non-B (NALL-1) and T-ALL (TALL-1) cells recognized B cell antigens distinct from Ia-like antigens on both normal and neoplastic B cells. After further absorption with tonsillar cells or normal B cell line (KO-HL-3), it reacted only with BALL-1 cells and did not react with other leukemia/lymphoma and normal B cell lines. The serum absorbed with tonsillar cells reacted only with BALL-1 and some B cell lines. Thus we were able to obtain antisera with specificity to B cell antigen, B-ALL antigen, and B cell line antigen.
通过静脉注射B细胞急性淋巴细胞白血病（ALL）细胞系（BALL-1）的活细胞，在白兔体内制备抗血清。经适当吸收后，通过膜免疫荧光检测抗血清对各种淋巴细胞系的反应性。用非T、非B（NALL-1）和T-ALL（TALL-1）细胞吸收的血清识别正常和肿瘤性B细胞上不同于Ia样抗原的B细胞抗原。用扁桃体细胞或正常B细胞系（KO-HL-3）进一步吸收后，它仅与BALL-1细胞反应，而不与其他白血病/淋巴瘤和正常B细胞系反应。用扁桃体细胞吸收的血清仅与BALL-1和一些B细胞系反应。因此，我们能够获得对B细胞抗原、B-ALL抗原和B细胞系抗原有特异性的抗血清。
