Tanigaki N, Tosi R, Centis D, Ferrara G B, Pressman D
Hum Immunol. 1981 Oct;3(2):93-108. doi: 10.1016/0198-8859(81)90047-1.
Peripheral lymphocytes from a panel of individuals who had been assayed for DR specificities by the conventional cytotoxicity assay were typed for DR "supertypic" specificities, DC1 and BR4 x 7, by the radioimmunoassay. A positive and a negative population were clearly distinguished for both specificities and the strong association of the DC1 specificity with DR1, 2, and w6 was confirmed as well as the BR4 x 7 specificity with DR4 and 7. Family study also supported this strong association. Appropriate papain digestion separated molecules carrying DC1 determinant from those carrying DR2 as well as from those carrying DRw6, and separated molecules carrying BR4 x 7 from those carrying DR4. Specificity analysis of the 8th Workshop antisera by use of these separated antigen preparations showed that some anti-DC1 antisera do not possess appreciable anti-DR1, 2, or w6 activity and vice versa. The same was found for DR4 x 7 in its relationship with DR4 and 7. The existing evidence could be explained most economically by assuming a genetic model of two loci in linkage disequilibrium each coding for analogous but distinct forms of the small (beta) subunits of Ia molecules.
通过放射免疫测定法，对一组经传统细胞毒性测定法检测过DR特异性的个体的外周淋巴细胞进行DR“超型”特异性DC1和BR4×7分型。两种特异性均能明确区分阳性和阴性群体，DC1特异性与DR1、2和w6的强关联性以及BR4×7特异性与DR4和7的强关联性均得到证实。家系研究也支持这种强关联性。适当的木瓜蛋白酶消化可将携带DC1决定簇的分子与携带DR2以及携带DRw6的分子分开，也可将携带BR4×7的分子与携带DR4的分子分开。使用这些分离的抗原制剂对第8届研讨会抗血清进行特异性分析表明，一些抗DC1抗血清不具有明显的抗DR1、2或w6活性，反之亦然。DR4×7与DR4和7的关系也是如此。现有证据可以通过假定一个遗传模型来最经济地解释，该模型中两个处于连锁不平衡状态的基因座各自编码Ia分子小（β）亚基的类似但不同形式。
