通过51Cr-LAI试验和EM试验检测小鼠肉瘤中肿瘤相关抗原（TAA）和胎儿抗原（FA）的异质性和特异性。
Heterogenicity and specificity of tumor associated antigens (TAA) and fetal antigens (FA) as detected by 51Cr-LAI test and EM test in mouse sarcomas.
作者信息
Werner H, Schulze H A
出版信息
Arch Geschwulstforsch. 1981;51(6):481-4.
Soluble extracts (3M KCl) were prepared from tumor tissue and mouse fetuses. After separation of ULTROGEL AcA 34 single fractions of these antigens were incubated with spleen cells of tumor bearing, respectively pregnant mice and the response was detected by 51Cr-LAI and/or EM techniques. There was only a partial correlation between the results of both test systems. Different antigen activities were found in three regions of molecular weight: P1 (200,000-250,000), P2 (120,000-160,000) and P3 (50,000-60,000). Individual specific activity was detected in P2 fractions of TAA whereas P3 fractions of TAA and FA as well as P1 fraction of FA revealed cross reactivities. Pregnant specificity was localized in P2 fraction of FA.
从肿瘤组织和小鼠胎儿制备可溶性提取物（3M KCl）。在分离ULTROGEL AcA 34后，将这些抗原的单一馏分分别与荷瘤小鼠和怀孕小鼠的脾细胞一起孵育，并通过51Cr-LAI和/或EM技术检测反应。两个测试系统的结果之间只有部分相关性。在分子量的三个区域发现了不同的抗原活性：P1（200,000 - 250,000）、P2（120,000 - 160,000）和P3（50,000 - 60,000）。在肿瘤相关抗原（TAA）的P2馏分中检测到个体特异性活性，而TAA和胎儿抗原（FA）的P3馏分以及FA的P1馏分显示出交叉反应性。怀孕特异性定位于FA的P2馏分中。
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