Harada H, Ueda O, Kochi F, Kobayashi T, Komazawa M
Gastroenterol Jpn. 1981 Dec;16(6):623-6. doi: 10.1007/BF02813799.
In order to investigate the cause of an increase in viscosity of pancreatic juice in patients with chronic pancreatitis, we performed comparative studies on voscosity and concentration of protein, hexosamine, three hydrolytic enzymes (amylase, chymotrypsinogen and lipase), calcium and gamma-Glutamyltranspeptidase in pure pancreatic juice. Pure pancreatic juice (PPJ) was obtained from seven control subjects, 10 patients with probable chronic pancreatitis and six patients with definite chronic pancreatitis by means of endoscopic retrograde catheterization of the papilla. Specimens were collected in ice-cooled tubes during the initial four-minute period and thereafter at two-minute intervals for 20 minutes after a rapid intravenous injection of secretin (Eisai Secrepan, 1 U/kg of body weight) and then at one-minute intervals for 10 minutes after a rapid intravenous injection of pancreozymin (Boots, 1 U/kg of body weight). It was demonstrated that the viscosity of PPJ was closely related to the concentration of hydrolytic enzyme protein, hexosamine and the presence of mucous substance and protein plugs.
为了探究慢性胰腺炎患者胰液黏度增加的原因，我们对纯胰液的黏度以及蛋白质、氨基己糖、三种水解酶（淀粉酶、胰凝乳蛋白酶原和脂肪酶）、钙和γ-谷氨酰转肽酶的浓度进行了对比研究。通过对乳头进行内镜逆行插管，从7名对照受试者、10名疑似慢性胰腺炎患者和6名确诊慢性胰腺炎患者中获取了纯胰液（PPJ）。在快速静脉注射促胰液素（卫材促胰液素，1 U/kg体重）后的最初4分钟内，将标本收集于冰冷却的试管中，此后每隔2分钟收集一次，共收集20分钟，然后在快速静脉注射胰酶泌素（布茨，1 U/kg体重）后每隔1分钟收集一次，共收集10分钟。结果表明，PPJ的黏度与水解酶蛋白、氨基己糖的浓度以及黏液物质和蛋白栓的存在密切相关。
