Magro A M
Int J Immunopharmacol. 1982;4(1):15-20. doi: 10.1016/0192-0561(82)90004-2.
Inhibitors of arachidonic acid metabolism were tested for their ability to block histamine release from human basophils. Eighteen inhibitors of lipoxygenases, cyclo-oxygenases, prostaglandin isomerases and thromboxane synthetases were tested. Agents inhibitory to the activity of lipoxygenases were effective blockers of IgE and non-IgE-mediated histamine release; agents antagonistic to cyclo-oxygenases, isomerases and thromboxane synthetases were not. These findings indicate that a functioning lipoxygenase pathway is essential for basophil activation and secretion and that the cyclo-oxygenase, isomerase and thromboxane synthetase pathways are not. Compounds antagonistic to phospholipase A also block histamine release, as does the intracellular Ca2+ antagonist TMB-8. The data are consistent with the idea that mast-cell and basophil activation and secretion involve phospholipase A generation of arachidonic acid, which is metabolized via the lipoxygenase pathway.
对花生四烯酸代谢抑制剂阻断人嗜碱性粒细胞释放组胺的能力进行了测试。测试了18种脂氧合酶、环氧化酶、前列腺素异构酶和血栓素合成酶的抑制剂。抑制脂氧合酶活性的药物是IgE和非IgE介导的组胺释放的有效阻断剂；对环氧化酶、异构酶和血栓素合成酶有拮抗作用的药物则不是。这些发现表明，正常运作的脂氧合酶途径对于嗜碱性粒细胞的激活和分泌至关重要，而环氧化酶、异构酶和血栓素合成酶途径则不然。对磷脂酶A有拮抗作用的化合物也能阻断组胺释放，细胞内Ca2+拮抗剂TMB-8也是如此。这些数据与肥大细胞和嗜碱性粒细胞的激活和分泌涉及磷脂酶A生成花生四烯酸并通过脂氧合酶途径进行代谢的观点一致。
