Mitchell E B, Askenase P W
J Exp Med. 1982 Jul 1;156(1):159-72. doi: 10.1084/jem.156.1.159.
Guinea pigs immunized with protein antigens emulsified with complete Freund's adjuvant (CFA) and skin tested at 3-4 wk have classical tuberculin-type delayed hypersensitivity (DH) reactions with few basophils present. However, recipients of T cells from these animals have delayed responses containing large basophil infiltrates and thus resemble basophil-rich cutaneous basophil hypersensitivity (CBH) responses that are elicited in animals immunized without CFA. This suggests that animals immunized with CFA have T cells with basophil-recruiting capacity but that this activity is suppressed. Using a transfer system, we found that immune serum from donors immunized with CFA had the ability to suppress the basophil-recruiting capacity of immune T cells. When immune serum and peritoneal exudate cells from guinea pigs immunized with CFA were co-transferred intravenously to normal recipients, the cell-mediated transfer of basophil-rich responses was suppressed. The responsible serum factor was antigen nonspecific, had an approximately 70,000 mol wt, and acted preferentially on cells from donors that express basophil-poor DH responses. Thus, tuberculin-type delayed hypersensitivity and CBH might be mediated by a common T cell, but the resulting basophil component of the delayed response depends on the modulation of T cell recruitment of basophils by factors in CFA-immune serum.
用完全弗氏佐剂（CFA）乳化的蛋白质抗原免疫的豚鼠，在3 - 4周时进行皮肤试验，会出现典型的结核菌素型迟发型超敏反应（DH），且几乎没有嗜碱性粒细胞。然而，来自这些动物的T细胞受体有延迟反应，其中含有大量嗜碱性粒细胞浸润，因此类似于在未用CFA免疫的动物中引发的富含嗜碱性粒细胞的皮肤嗜碱性粒细胞超敏反应（CBH）。这表明用CFA免疫的动物具有具有嗜碱性粒细胞招募能力的T细胞，但这种活性受到抑制。使用转移系统，我们发现用CFA免疫的供体的免疫血清有能力抑制免疫T细胞的嗜碱性粒细胞招募能力。当将用CFA免疫的豚鼠的免疫血清和腹腔渗出细胞静脉内共同转移到正常受体时，富含嗜碱性粒细胞反应的细胞介导转移受到抑制。起作用的血清因子是非抗原特异性的，分子量约为70,000，并且优先作用于来自表达嗜碱性粒细胞少的DH反应的供体的细胞。因此，结核菌素型迟发型超敏反应和CBH可能由共同的T细胞介导，但迟发型反应中产生的嗜碱性粒细胞成分取决于CFA免疫血清中的因子对T细胞招募嗜碱性粒细胞的调节。
