犬轮状病毒的分离与鉴定
Isolation and characterization of a canine rotavirus.
作者信息
Hoshino Y, Wyatt R G, Scott F W, Appel M J
出版信息
Arch Virol. 1982;72(1-2):113-25. doi: 10.1007/BF01314456.
Canine rotavirus particles were visualized by direct electron microscopy in the feces from a clinically normal dog. The virus was subsequently propagated in cell cultures; it was characterized and compared with rotaviruses from other species. Replication of the virus in cell culture was found to be less dependent upon trypsin than that of human, bovine and porcine rotaviruses. Reproducible, sharp-edged plaques of various sizes were produced by the canine rotavirus in an established cell line of fetal rhesus monkey kidney, MA 104, under overlays of carboxymethyl cellulose or agarose. Intracytoplasmic inclusion bodies of different sizes and shapes were produced in infected MA 104 cells. By plaque reduction neutralization assay, a two-way antigenic relationship was found between the canine (CU-1) and simian (rhesus MMU 18006 and SA-11) rotaviruses. The canine rotavirus had a one-way antigenic relationship with feline (Taka), bovine (NCDV), and porcine (OSU) rotaviruses.
通过直接电子显微镜观察，在一只临床健康犬的粪便中发现了犬轮状病毒颗粒。随后该病毒在细胞培养物中进行增殖；对其进行了特性鉴定，并与来自其他物种的轮状病毒进行了比较。发现该病毒在细胞培养中的复制比人、牛和猪轮状病毒对胰蛋白酶的依赖性更低。在羧甲基纤维素或琼脂糖覆盖下，犬轮状病毒在恒河猴胎儿肾的既定细胞系MA 104中产生了大小各异、边缘清晰可重复的蚀斑。在感染的MA 104细胞中产生了不同大小和形状的胞质内包涵体。通过蚀斑减少中和试验，发现犬（CU-1）轮状病毒与猿猴（恒河猴MMU 18006和SA-11）轮状病毒之间存在双向抗原关系。犬轮状病毒与猫（Taka）、牛（NCDV）和猪（OSU）轮状病毒存在单向抗原关系。
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