与小核RNA U1互补的小鼠DNA序列。

Mouse DNA sequences complementary to small nuclear RNA U1.

作者信息

Piechaczyk M, Lelay-Taha M N, Sri-Widada J, Brunel C, Liautard J P, Jeanteur P

出版信息

Nucleic Acids Res. 1982 Aug 11;10(15):4627-40. doi: 10.1093/nar/10.15.4627.

DOI:10.1093/nar/10.15.4627

PMID:6182525

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC321117/

Abstract

A mouse genomic library was screened for sequences complementary to U1 nuclear RNA. Out of the eight clones tested, none contained more than one copy of U1. Six of them were identical and one of those (clone 0U1-XIII) was further analyzed. This latter clone contained no other gene for discrete species of small size RNA in the 8 Kb EcoRI fragment encoding U1. A 248 bp Bg1II fragment from 0U1-XIII encompassing the full length of U1 as well as flanking regions on both sides has been subcloned and sequenced in M13 phage. Although the coding region was 96.5% homologous to rat U1a RNA, there is no direct evidence that this clone is a true gene. 3' and 5' flanking sequences of this as well as other published clones have been searched for homologies and the results of this search are discussed.

摘要

对小鼠基因组文库进行筛选，寻找与U1核RNA互补的序列。在测试的八个克隆中，没有一个克隆包含超过一份的U1。其中六个是相同的，对其中一个（克隆0U1-XIII）进行了进一步分析。后一个克隆在编码U1的8 Kb EcoRI片段中不包含其他离散小尺寸RNA物种的基因。一个来自0U1-XIII的248 bp Bg1II片段，包含U1的全长以及两侧的侧翼区域，已被亚克隆并在M13噬菌体中测序。尽管编码区与大鼠U1a RNA有96.5%的同源性，但没有直接证据表明这个克隆是一个真正的基因。已经搜索了该克隆以及其他已发表克隆的3'和5'侧翼序列的同源性，并讨论了搜索结果。