Billing R, Rafizadeh B, Drew I, Hartman G, Gale R, Terasaki P
J Exp Med. 1976 Jul 1;144(1):167-78. doi: 10.1084/jem.144.1.167.
A previously uncharacterized human B-lymphocyte antigen has been detected by rabbit antisera raised to papain digests of spleen cell membranes. The unabsorbed sera reacted in both cytotoxicity and immunofluorescent tests with normal B lymphocytes and cultured B-cell lines but not with normal T lymphocytes or cultured T-cell lines. The cytotoxicity titers against B cells were as high as 1:32,000, whereas the same sera undiluted were negative against T cells. By immunofluorescent staining 6-14% of unfractionated normal lymphocytes and 48-85% of B-rich lymphocyte preparations were positive. Normal peripheral blood granulocytes, platelets, erythrocytes, and phytohemagglutinin blasts were negative. The antisera reacted with the same high titers against leukemia cells from approximately 70% of the patients with acute lymphocytic leukemia, acute myelocytic leukemia, chronic myelocytic leukemia, and seven of eight cases of chronic lymphocytic leukemia. From absorption studies it appeared that the same antigen was being expressed by leukemia cells and normal B lymphocytes. Using immunofluorescent staining the anti-B-cell antisera were able to detect positive leukemia cells in the bone marrow of patients with advanced leukemia and to monitor the elimination of these cells after chemotherapy. Soluble B-cell antigen was found in the serum of some leukemia and lymphoma patients do but not in normal serum.
用针对脾细胞膜木瓜蛋白酶消化物产生的兔抗血清检测到了一种以前未被鉴定的人类B淋巴细胞抗原。未吸收的血清在细胞毒性和免疫荧光试验中与正常B淋巴细胞及培养的B细胞系发生反应，但与正常T淋巴细胞或培养的T细胞系不发生反应。针对B细胞的细胞毒性效价高达1:32,000，而相同的未稀释血清对T细胞呈阴性。通过免疫荧光染色，6 - 14%的未分级正常淋巴细胞和48 - 85%的富含B细胞的淋巴细胞制剂呈阳性。正常外周血粒细胞、血小板、红细胞和植物血凝素刺激的母细胞呈阴性。这些抗血清以相同的高效价与大约70%的急性淋巴细胞白血病、急性髓细胞白血病、慢性髓细胞白血病患者以及8例慢性淋巴细胞白血病中的7例患者的白血病细胞发生反应。从吸收研究来看，白血病细胞和正常B淋巴细胞似乎表达相同的抗原。使用免疫荧光染色，抗B细胞抗血清能够在晚期白血病患者的骨髓中检测到阳性白血病细胞，并监测化疗后这些细胞的清除情况。在一些白血病和淋巴瘤患者的血清中发现了可溶性B细胞抗原，但正常血清中未发现。
