Rosan B, Argenbright L
Infect Immun. 1982 Dec;38(3):925-31. doi: 10.1128/iai.38.3.925-931.1982.
Previous studies indicated that the teichoic acid isolated from strains of Streptococcus sanguis was group specific and defined the Lancefield group H streptococci. To determine the specific antigenic determinants, the antigen was extracted from a group H streptococcus (ATCC 903) by the phenol-water method and purified by column chromatography. The isolated antigen had a glycerol/phosphate/glucose molar ratio of 1:0.9:0.3; the lipid concentration was 7.6% of its dry weight. No nucleic acids were detected, and amino acids constituted approximately 2% of the dry weight. The minimum concentration of antigen required to sensitize erythrocytes for hemagglutination with a 1:1,000 dilution of either group H antiserum or antiteichoic acid serum was 0.02 microgram/ml. Hemagglutination inhibition studies suggested that the major antigenic determinant consisted of an alpha-glucose linked to the glycerol phosphate backbone.
先前的研究表明，从血链球菌菌株中分离出的磷壁酸具有群特异性，并可界定兰斯菲尔德H群链球菌。为确定具体的抗原决定簇，采用酚-水法从一株H群链球菌（ATCC 903）中提取抗原，并通过柱色谱法进行纯化。分离出的抗原甘油/磷酸盐/葡萄糖的摩尔比为1:0.9:0.3；脂质浓度占其干重的7.6%。未检测到核酸，氨基酸约占干重的2%。用H群抗血清或抗磷壁酸血清1:1000稀释液使红细胞致敏以进行血凝反应所需的最低抗原浓度为0.02微克/毫升。血凝抑制研究表明，主要抗原决定簇由连接在甘油磷酸主链上的α-葡萄糖组成。
