Valesini G, Tincani A, Harris E N, Mantelli P G, Allegri F, Palmieri G, Hughes G R, Balsano F, Balestrieri G
Istituto di Clinica Medica I, Universitá di Roma La Sapienza, Italy.
Clin Exp Immunol. 1987 Oct;70(1):18-25.
Using hybridoma technology we produced monoclonal antibodies (MoAb) to idiotypic determinants on human anti-cardiolipin antibodies purified from a patient with SLE. Hybridomas were screened by inhibition of cardiolipin binding activity of sera from patients with SLE. Seven hybridomas were selected, two of which were studied extensively. Sera from a number of patients with SLE were found to share idiotypic determinants. This cross-reacting idiotype was not detectable on anti-cardiolipin antibodies in syphilis sera. The cross-reacting idiotype was present in sera with anti-ssDNA antibodies even though some of these sera had no anti-cardiolipin antibodies. We propose that these MoAb may recognize a regulatory idiotype.
我们利用杂交瘤技术制备了针对从一名系统性红斑狼疮（SLE）患者纯化的人抗心磷脂抗体独特型决定簇的单克隆抗体（MoAb）。通过抑制SLE患者血清的心磷脂结合活性来筛选杂交瘤。挑选出了7个杂交瘤，其中2个进行了深入研究。发现许多SLE患者的血清具有共同的独特型决定簇。在梅毒血清的抗心磷脂抗体上未检测到这种交叉反应独特型。即使其中一些血清没有抗心磷脂抗体，具有抗单链DNA（ssDNA）抗体的血清中也存在交叉反应独特型。我们提出这些单克隆抗体可能识别一种调节性独特型。
