Sidiropoulou-Skokou S A, Issidorides M R
Arch Pathol Lab Med. 1983 May;107(5):270-3.
Histological lesions in man, presumably indicating kidney damage, may occur during therapy with lithium carbonate. We studied the cellular effects of lithium carbonate applied in vitro to slices of kidney from adult female Sprague-Dawley rats. Alternate slices were immersed in saline solution containing 1.1 mEq/L of lithium carbonate; the other slices were immersed in ordinary saline solution as controls. Compared with control specimens, a two-hour incubation in lithium carbonate solution showed (1) with toluidine blue 0, in the cells of the collecting ducts a fibrous metachromatic network that was interpreted as indicating microtubule bundles; and (2) with Brookes' stain in the proximal convolutions, increased acidophilia of the hyaline droplets and of the nucleus that implied interaction of lithium carbonate with the protein transport and degradation mechanism located in these cells. The results can be interpreted as being due to polymerizations or to conformational changes of protein components caused by lithium. These changes are consistent with altered functions of the kidney observed in manic-depressive patients receiving treatment with lithium carbonate, which lead to polyuria (collecting ducts) and may lead to nephrotoxicity (proximal convolutions).
在使用碳酸锂治疗期间，人体可能会出现组织学病变，这大概表明肾脏受到了损害。我们研究了将碳酸锂体外应用于成年雌性斯普拉格 - 道利大鼠肾脏切片后的细胞效应。将交替的切片浸入含有1.1毫当量/升碳酸锂的盐溶液中；其他切片浸入普通盐溶液作为对照。与对照标本相比，在碳酸锂溶液中孵育两小时后显示：（1）用甲苯胺蓝O染色，在集合管细胞中出现纤维状异染网络，这被解释为表明存在微管束；（2）用布鲁克斯染色法观察近端曲管，透明滴和细胞核的嗜酸性增加，这意味着碳酸锂与这些细胞中的蛋白质转运和降解机制发生了相互作用。这些结果可以解释为是由锂引起的蛋白质成分的聚合或构象变化所致。这些变化与接受碳酸锂治疗的躁狂抑郁症患者中观察到的肾脏功能改变一致，这些改变会导致多尿（集合管），并可能导致肾毒性（近端曲管）。
