颗粒计数免疫测定法（PACIA）在特定抗体滴定中的应用。应用于蜜蜂毒液脱敏患者的血清。

The use of particle counting immunoassay (PACIA) for the titration of specific antibodies. Application to sera from honeybee venom-desensitized patients.

作者信息

Magnusson C G, Djurup R, Weeke B, Masson P L

出版信息

Int Arch Allergy Appl Immunol. 1983;71(2):144-50. doi: 10.1159/000233378.

Abstract

Particle counting immunoassay (PACIA) is shown to be a suitable nonradioisotopic method for the titration of anti-phospholipase A2 antibodies in the sera from honeybee venom-desensitized patients. The sensitivity of the method is equal to the RAST-IgG with a correlation coefficient between the two methods of r = 0.94. Within-day and between-day precisions were 2.5 and 9.2%, respectively. The main advantages of PACIA are: (i) the use of easily prepared non-radioactive reagents having a shelf-life of at least 1 year at -20 degrees C: (ii) partial automation; (iii) calibration with antisera of animal origin, and (iv) the possibility of allergen standardization of honeybee venom extracts.

摘要

颗粒计数免疫分析（PACIA）被证明是一种适用于滴定蜜蜂毒液脱敏患者血清中抗磷脂酶A2抗体的非放射性同位素方法。该方法的灵敏度与放射性变应原吸附试验-IgG相当，两种方法之间的相关系数r = 0.94。日内精密度和日间精密度分别为2.5%和9.2%。PACIA的主要优点包括：（i）使用易于制备的非放射性试剂，在-20℃下保质期至少为1年；（ii）部分自动化；（iii）用动物源性抗血清进行校准；以及（iv）对蜜蜂毒液提取物进行过敏原标准化的可能性。