Djurup R, Magnusson C G, Minuva U, Søndergaard I, Osterballe O, Weeke B
Allergy. 1983 Apr;38(3):173-82. doi: 10.1111/j.1398-9995.1983.tb01603.x.
An automated particle counting immunoassay (PACIA) for measurement of blocking antibodies (antigen neutralizing capacity) against timothy grass pollen extract in sera from desensitized allergics is described. Latex particles coated with F(ab')2-anti-timothy are agglutinated by timothy. Serum containing anti-timothy antibodies inhibits the agglutination. Non-agglutinated particles are counted in a modified AutoCounter. Nineteen of 20 sera from timothy allergics who had undergone immunotherapy with purified timothy extract for 30 weeks, showed significant agglutination-inhibition. None of 42 normal human sera gave significant inhibition. The inhibiting antibody could be removed by absorption with protein A and was thus of non-IgE nature, i.e. blocking antibody. The results obtained correlated statistically significantly with those found with a double-antibody method (rS = 0.62, n = 20, t = 3.35, P less than 0.01) and with the cumulated dosage of timothy allergen extract administered to the individual patient (rS = 0.56, n = 20, t = 2.87, P less than 0.02). Between-assay coefficient of variation was from 6.4% to 18.3%. The capacity is 40 samples per hour. The method has also been applied to measurement of blocking antibodies to honey bee and wasp venom.
本文描述了一种用于检测脱敏过敏患者血清中针对梯牧草花粉提取物的封闭抗体（抗原中和能力）的自动化颗粒计数免疫测定法（PACIA）。包被有抗梯牧草F(ab')2的乳胶颗粒可被梯牧草凝集。含有抗梯牧草抗体的血清可抑制凝集反应。未凝集的颗粒在改良的自动计数器中进行计数。20名接受纯化梯牧草提取物免疫治疗30周的梯牧草过敏患者中，有19人的血清显示出显著的凝集抑制作用。42份正常人血清均未产生显著抑制作用。抑制性抗体可通过与蛋白A吸附而去除，因此具有非IgE性质，即封闭抗体。所得结果与双抗体法所得结果在统计学上显著相关（rS = 0.62，n = 20，t = 3.35，P < 0.01），并与给予个体患者的梯牧草过敏原提取物的累积剂量相关（rS = 0.56，n = 20，t = 2.87，P < 0.02）。批间变异系数为6.4%至18.3%。检测能力为每小时40个样本。该方法也已应用于检测针对蜜蜂和黄蜂毒液的封闭抗体。
