Jacobsen P L, Greenspan J S
J Immunopharmacol. 1982;4(4):355-65. doi: 10.3109/08923978209019453.
Isoprinosine has been reported to stimulate immunological responses involving different cell types. This study attempted to identify which cell type responds to isoprinosine in the blast transformation assay using the mitogen phytohemagglutinin (PHA). Human peripheral blood mononuclear cells, and the same cell population depleted of monocytes, were used to determine whether the absence of monocytes alters the response to isoprinosine. To determine whether there is a critical period of time during the assay when isoprinosine has its effect, the cells were selectively preexposed to drug and/or mitogen for a limited period of time, then cultured. Depletion of monocytes in itself augmented the response to mitogen, but the response could still be potentiated by isoprinosine, indicating that the drug's effect was on cells other than monocytes. Preexposure to isoprinosine and/or PHA, followed by culture with PHA with or without isoprinosine, showed that PHA initiates transformation within the first 3 hours and that initiation, a monocyte function, is not sensitive to isoprinosine. Both approaches suggest that isoprinosine does not affect monocytes and that it probably acts on T cells to produce its stimulatory effect in this in vitro assay.
据报道，异丙肌苷可刺激涉及不同细胞类型的免疫反应。本研究试图在使用促有丝分裂原植物血凝素（PHA）的 blast 转化试验中确定哪种细胞类型对异丙肌苷有反应。使用人外周血单核细胞以及去除单核细胞的相同细胞群体，以确定单核细胞的缺失是否会改变对异丙肌苷的反应。为了确定在试验过程中异丙肌苷发挥作用是否存在关键时间段，将细胞在有限时间段内选择性地预先暴露于药物和/或促有丝分裂原，然后进行培养。单核细胞的去除本身增强了对促有丝分裂原的反应，但异丙肌苷仍可增强该反应，表明该药物的作用对象是单核细胞以外的细胞。预先暴露于异丙肌苷和/或PHA，然后用含或不含异丙肌苷的PHA进行培养，结果表明PHA在前3小时内启动转化，并且启动这一单核细胞功能对异丙肌苷不敏感。这两种方法均表明异丙肌苷不影响单核细胞，并且在该体外试验中它可能作用于T细胞以产生其刺激作用。
