Kuhajda F P, Offutt L E, Mendelsohn G
Cancer. 1983 Oct 1;52(7):1257-64. doi: 10.1002/1097-0142(19831001)52:7<1257::aid-cncr2820520721>3.0.co;2-6.
Carcinoembryonic antigen (CEA) has been shown to be a useful tumor marker in patients with breast carcinoma. The unlabeled antibody immunoperoxidase technique was used to localize CEA in 93 cases of primary breast carcinoma, 15 cases of atypical duct papillomatosis, and 4 cases of duct papilloma. Normal breast epithelium and breast epithelium in fibrocystic disease did not stain positively for CEA. Twenty-four of 27 (88%) intraductal carcinomas, and 47 of 69 (68%) infiltrating duct carcinomas were CEA positive. In contrast, only 5 of 21 (23%) in situ lobular carcinomas and 8 of 24 (33%) infiltrating lobular carcinomas were positive for CEA. All 15 cases of atypical epithelial papillomatosis were negative, whereas 1 of the 4 cases of duct papilloma exhibited microscopic foci of weak CEA positivity. There was a trend for infiltrating duct carcinomas, 3 cm in diameter or smaller, staining strongly positive for CEA, to be associated with synchronous axillary lymph node metastases (P = 0.09). Tumor heterogeneity was a constant feature of CEA staining with positivity varying from region to region and even from cell to cell. Positive immunohistochemical staining for CEA may play an adjunctive role in discriminating intraductal carcinoma from atypical papillary ductal proliferations.
癌胚抗原（CEA）已被证明是乳腺癌患者有用的肿瘤标志物。采用未标记抗体免疫过氧化物酶技术对93例原发性乳腺癌、15例非典型导管乳头状瘤病和4例导管乳头状瘤中的CEA进行定位。正常乳腺上皮和纤维囊性疾病中的乳腺上皮CEA染色均为阴性。27例导管内癌中有24例（88%）、69例浸润性导管癌中有47例（68%）CEA呈阳性。相比之下，21例原位小叶癌中只有5例（23%）、24例浸润性小叶癌中只有8例（33%）CEA呈阳性。15例非典型上皮乳头状瘤病均为阴性，而4例导管乳头状瘤中有1例在显微镜下显示出微弱的CEA阳性灶。直径3厘米或更小的浸润性导管癌CEA染色强阳性，有与同期腋窝淋巴结转移相关的趋势（P = 0.09）。肿瘤异质性是CEA染色的一个恒定特征，阳性程度因区域甚至细胞而异。CEA免疫组化阳性染色在鉴别导管内癌与非典型乳头状导管增生方面可能起辅助作用。
