Domagala W, Markiewski M, Kubiak R, Bartkowiak J, Osborn M
Department of Pathology, Medical Academy, Szczecin, Poland.
Virchows Arch A Pathol Anat Histopathol. 1993;423(6):497-502. doi: 10.1007/BF01606541.
Vimentin, p53 protein and cathepsin D positivity were assessed by immunohistochemistry, and oestrogen receptor (ER) by an enzyme immunoassay, in invasive lobular carcinomas (LC) of the breast. While vimentin was positive in only 5% (3/57) and p53 protein was positive only in 3% (2/63), cathepsin D was expressed in 86% (48/56) and ER in 78% (25/32). Classical LC were negative for p53 protein and all except one were cathepsin D positive. These results are in contrast to invasive ductal breast carcinomas (DC), where the reported average incidence of vimentin and p53 protein is much higher (19% and 33% respectively) and that of cathepsin D and ER lower (63% and 67% respectively). Thus lack of expression of vimentin and lack of p53 positivity together with high incidence of expression of cathepsin D and ER are more often associated with lobular than with ductal differentiation of invasive breast cancer. The results show that LC, distinguished morphologically, can further be defined by its immunohistochemical profile. This in turn may point to underlying biological differences between LC and DC.
采用免疫组织化学法评估波形蛋白、p53蛋白和组织蛋白酶D的阳性表达情况，采用酶免疫分析法评估雌激素受体（ER）的表达情况，研究对象为乳腺浸润性小叶癌（LC）。波形蛋白仅在5%（3/57）的病例中呈阳性，p53蛋白仅在3%（2/63）的病例中呈阳性，而组织蛋白酶D在86%（48/56）的病例中表达，ER在78%（25/32）的病例中表达。经典型LC的p53蛋白呈阴性，除1例之外其余所有病例的组织蛋白酶D均呈阳性。这些结果与乳腺浸润性导管癌（DC）相反，在DC中，波形蛋白和p53蛋白的报道平均发生率要高得多（分别为19%和33%），而组织蛋白酶D和ER的发生率较低（分别为63%和67%）。因此，波形蛋白表达缺失、p53阴性以及组织蛋白酶D和ER高表达更常与浸润性乳腺癌的小叶分化而非导管分化相关。结果表明，形态学上区分的LC可通过其免疫组化特征进一步定义。这反过来可能表明LC和DC之间存在潜在的生物学差异。
