Wangel A G, Kontiainen S
Clin Exp Immunol. 1983 Aug;53(2):451-7.
Lymphocytes from normal human donors were cultured in Marbrook flasks in the presence of C-reactive protein (CRP), concanavalin A (Con A) or serum amyloid A protein low molecular weight component (SAAL) and the culture supernatants were tested for their ability to suppress the pokeweed mitogen (PWM) driven response of normal human lymphocytes. The supernatants from CRP and Con A stimulated lymphocytes, when added to cultures at initiation at a final concentration of 5%, suppressed the plaque forming cell (PFC) response in the three major immunoglobulin (Ig) classes. The suppressive effect could be removed by absorption of the culture supernatants with lentil lectin and with antisera to antigen specific helper and suppressor factors and could be recovered in the eluate. The results indicate that CRP stimulated cells exert their suppressive effect through a soluble mediator(s) which is similar to Con A generated suppressor factor(s) in respect to its time of action, its effect on the Ig classes M, G and A, its lack of cytoxicity and some structural aspects.
将来自正常人类供体的淋巴细胞置于马尔布鲁克培养瓶中，在存在C反应蛋白（CRP）、伴刀豆球蛋白A（Con A）或血清淀粉样蛋白A低分子量成分（SAAL）的情况下进行培养，然后检测培养上清液抑制正常人淋巴细胞受商陆有丝分裂原（PWM）驱动反应的能力。当CRP和Con A刺激的淋巴细胞培养上清液以5%的终浓度在培养开始时加入培养物中时，可抑制三种主要免疫球蛋白（Ig）类别的空斑形成细胞（PFC）反应。通过用扁豆凝集素以及针对抗原特异性辅助因子和抑制因子的抗血清吸收培养上清液，可消除这种抑制作用，且在洗脱液中可恢复。结果表明，CRP刺激的细胞通过一种可溶性介质发挥其抑制作用，该介质在作用时间、对Ig M、G和A类别的影响、缺乏细胞毒性以及一些结构方面与Con A产生的抑制因子相似。
