大鼠心室和心房肌体外蛋白质合成速率及RNA含量的差异
Differential rates of protein synthesis in vitro and RNA contents in rat heart ventricular and atrial muscle.
作者信息
Smith D M, Sugden P H
出版信息
Biochem J. 1983 Aug 15;214(2):497-502. doi: 10.1042/bj2140497.
Abstract
The rates of protein synthesis in perfused rat heart ventricular or atrial muscle were measured by incorporation of [U-14C]phenylalanine in the presence of the remaining plasma amino acids. Atrial protein-synthesis rates were about twice the ventricular rates. Atrial RNA contents were also about twice the ventricular contents. Thus the efficiencies of protein synthesis (protein-synthesis rate/RNA) in the two compartments were similar. There were marked differences in ventricular and atrial RNA contents during the course of rat growth. Atrial RNA content was always greater than ventricular content and declined more slowly during growth, producing a 2-fold change in atrial/ventricular RNA-content ratio between the 88 g and 370 g rat groups.
摘要
在灌注大鼠心脏的心室或心房肌中，通过在其余血浆氨基酸存在的情况下掺入[U-¹⁴C]苯丙氨酸来测量蛋白质合成速率。心房蛋白质合成速率约为心室速率的两倍。心房RNA含量也约为心室含量的两倍。因此，两个腔室中蛋白质合成的效率（蛋白质合成速率/RNA）相似。在大鼠生长过程中，心室和心房的RNA含量存在显著差异。心房RNA含量始终高于心室含量，并且在生长过程中下降得更慢，在88克和370克大鼠组之间，心房/心室RNA含量比发生了2倍的变化。
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