Hasegawa T, Ohta M, Mori M, Nakashima I, Kato N
Microbiol Immunol. 1983;27(8):683-94. doi: 10.1111/j.1348-0421.1983.tb00631.x.
In a series of our earlier studies, the O3 antigen isolated from culture supernatant of Klebsiella pneumoniae strain Kasuya (O3:K1) (KO3) was shown to exhibit very strong adjuvant activity in mice. KO3 obtained was homogeneous in analyses by either gel filtration or ultracentrifugation. Its molecular weight determined by ultracentrifugal analysis was greater than 2 X 10(6). It contained 37.9% C, 6.20% H, 0.24% N, and less than 0.1% P. KO3 was degraded into the polysaccharide moiety and lipid moiety (about 20%) by hydrolysis with 1% acetic acid at 100 C for 1 hr. The molecular weight of the polysaccharide moiety obtained by the hydrolysis was 16,200 as determined by the Somogyi-Nelson method. Chemical analyses using methylation analysis and Smith degradation as the principal methods indicated that the polysaccharide moiety consisted of a mannan which has a pentasaccharide repeating unit of alpha-mannosyl-1,3-alpha-mannosyl-1,2-alpha-mannosyl-1,2-alpha-mannosyl-1, 2-alpha-mannose joined through alpha-1,3-mannosyl linkages. The number of repetitions was less than 20. The fact that minor components such as 2-keto-3-deoxyoctonate and glucose were detected suggests the presence of a core oligosaccharide, but its precise structure is unknown.
在我们早期的一系列研究中，从肺炎克雷伯菌Kasuya菌株（O3:K1）（KO3）的培养上清液中分离出的O3抗原在小鼠中显示出非常强的佐剂活性。通过凝胶过滤或超速离心分析获得的KO3是均一的。通过超速离心分析测定其分子量大于2×10⁶。它含有37.9%的碳、6.20%的氢、0.24%的氮和少于0.1%的磷。KO3在100℃下用1%乙酸水解1小时后降解为多糖部分和脂质部分（约20%）。通过索莫吉 - 纳尔逊法测定，水解得到的多糖部分的分子量为16,200。以甲基化分析和史密斯降解为主要方法的化学分析表明，多糖部分由一种甘露聚糖组成，该甘露聚糖具有一个五糖重复单元，即α - 甘露糖基 - 1,3 - α - 甘露糖基 - 1,2 - α - 甘露糖基 - 1,2 - α - 甘露糖基 - 1,2 - α - 甘露糖，通过α - 1,3 - 甘露糖基键连接。重复次数少于20次。检测到2 - 酮 - 3 - 脱氧辛糖酸和葡萄糖等次要成分这一事实表明存在核心寡糖，但其精确结构未知。
