Larsson H O, Lorentzon R, Boquist L
Cell Tissue Res. 1984;235(1):51-8. doi: 10.1007/BF00213722.
Stereology and semi-automatic image analysis were used with the aim of comparing the structure of parathyroid glands from untreated adult Mongolian gerbils fixed by immersion with those fixed by perfusion. Subclassification of the chief cells based upon the staining affinity or electron density of the cytoplasm was readily performed only in glands fixed by immersion, and so-called atrophic cells were observed only in these glands. The atrophic cells were often surrounded by "light" chief cells. In glands fixed by perfusion, "light" chief cells were only rarely encountered. A significant difference between glands fixed by immersion and those fixed by perfusion was found only with regard to the form of cells and nuclei, those fixed by perfusion being more spherical. When comparing individual cells within glands fixed by immersion, "light" chief cells were more spherical and had a significantly larger nuclear and cellular size, and a lower mitochondrial volume density than the "intermediate"/"dark" chief cells. Otherwise there were no significant differences in any of the parameters investigated. These data indicate that occurrence of so-called "light" chief cells and atrophic cells is a result of improper fixation. The results of this study do not favour the concept of a functional cycle with a simultaneous occurrence of active and inactive cells within parathyroid glands.
采用体视学和半自动图像分析方法，旨在比较经浸入固定的未处理成年蒙古沙鼠甲状旁腺结构与经灌注固定的甲状旁腺结构。仅在经浸入固定的腺体中，能够依据细胞质的染色亲和力或电子密度对主细胞进行亚分类，并且仅在这些腺体中观察到所谓的萎缩细胞。萎缩细胞常被“浅色”主细胞包围。在经灌注固定的腺体中，很少见到“浅色”主细胞。浸入固定的腺体与灌注固定的腺体之间仅在细胞和细胞核形态方面存在显著差异，灌注固定的腺体更呈球形。在比较经浸入固定的腺体内的单个细胞时，“浅色”主细胞更呈球形，其细胞核和细胞大小显著更大，线粒体体积密度低于“中间型”/“深色”主细胞。否则，在所研究的任何参数中均无显著差异。这些数据表明，所谓“浅色”主细胞和萎缩细胞的出现是固定不当的结果。本研究结果不支持甲状旁腺内同时存在活跃细胞和非活跃细胞的功能循环概念。
