Tax W J, Tidman N, Janossy G, Trejdosiewicz L, Willems R, Leeuwenberg J, De Witte T J, Capel P J, Koene R A
Clin Exp Immunol. 1984 Feb;55(2):427-36.
WT 1, an IgG2a subclass monoclonal antibody, recognizes a human T lineage specific antigen (mol. wt 40,000). This antigen is strongly expressed on thymic T blasts, and on peripheral T cells activated by phytohaemagglutinin, whereas cortical thymocytes and peripheral T cells are moderately positive for WT 1. In contrast, B lymphocytes, myeloid and erythroid cells, including the progenitor cells of these lineages, and terminal deoxynucleotidyl transferase positive cells in the bone marrow, are all WT 1 negative. Binding of WT 1 to T cells is blocked by a previously described antibody (3A1) suggesting that both antibodies bind to the same antigen present on human T cells. WT 1, however, is also reactive with T lymphocytes from rhesus monkeys whereas 3A1 is not. Therefore, the biological effects of WT 1 could be studied in a monkey model. In a skin allograft model, WT 1 was immunosuppressive and induced a marked prolongation of graft survival.
WT 1是一种IgG2a亚类单克隆抗体，可识别一种人类T细胞谱系特异性抗原（分子量40,000）。该抗原在胸腺T母细胞以及由植物血凝素激活的外周T细胞上强烈表达，而皮质胸腺细胞和外周T细胞对WT 1呈中度阳性。相比之下，B淋巴细胞、髓系和红系细胞，包括这些谱系的祖细胞，以及骨髓中末端脱氧核苷酸转移酶阳性细胞，均为WT 1阴性。WT 1与T细胞的结合被先前描述的抗体（3A1）阻断，这表明两种抗体都与人类T细胞上存在的相同抗原结合。然而，WT 1也与恒河猴的T淋巴细胞发生反应，而3A1则不反应。因此，可以在猴模型中研究WT 1的生物学效应。在皮肤同种异体移植模型中，WT 1具有免疫抑制作用，并显著延长了移植物存活时间。
