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烟草和向日葵冠瘿瘤中与TR-DNA同源的五个转录本的图谱定位。

Map locations of five transcripts homologous to TR-DNA in tobacco and sunflower crown gall tumors.

作者信息

Winter J A, Wright R L, Gurley W B

出版信息

Nucleic Acids Res. 1984 Mar 12;12(5):2391-406. doi: 10.1093/nar/12.5.2391.

DOI:10.1093/nar/12.5.2391
PMID:6200831
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC318670/
Abstract

Polyadenylated RNA from two octopine type tumor lines (E1, PSCG-15955) was analyzed by RNA blot hybridization and shown to contain five major transcripts homologous to TR DNA. In tobacco E1 tissue, the molecular weights of the TR homologous RNAs are 1.65 kb, 1.55 kb, 1.45 kb, 1.05 kb, and 0.78 kb. The 5' - and 3'-termini of each of the five E1 tumor transcripts were determined by S1 nuclease hybrid protection mapping. The polarity of transcription for the 0.78, 1.05 and 1.65 kb RNAs is from left to right, while the 1.55 and 1.45 kb RNAs are transcribed from right to left. Although we see the same size transcripts in sunflower PSCG-15955 tissue (with the exception of a 0.89 kb RNA in place of the 1.55 kb transcript of E1) there is an apparent difference in the relative abundance of the various RNAs between the two tumor lines.

摘要

通过RNA印迹杂交分析了来自两个章鱼碱型肿瘤系（E1、PSCG - 15955）的聚腺苷酸化RNA，结果显示其含有五种与TR DNA同源的主要转录本。在烟草E1组织中，TR同源RNA的分子量分别为1.65 kb、1.55 kb、1.45 kb、1.05 kb和0.78 kb。通过S1核酸酶杂交保护图谱确定了E1肿瘤的这五种转录本各自的5'端和3'端。0.78 kb、1.05 kb和1.65 kb RNA的转录极性是从左到右，而1.55 kb和1.45 kb RNA是从右到左转录。尽管我们在向日葵PSCG - 15955组织中看到了相同大小的转录本（除了用0.89 kb RNA取代了E1的1.55 kb转录本），但这两个肿瘤系中各种RNA的相对丰度存在明显差异。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5769/318670/65879c81a334/nar00323-0171-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5769/318670/15c519a48450/nar00323-0167-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5769/318670/2a6788df8513/nar00323-0167-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5769/318670/620caaf85612/nar00323-0168-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5769/318670/d042594039ba/nar00323-0169-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5769/318670/0db2f4d07d9f/nar00323-0170-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5769/318670/65879c81a334/nar00323-0171-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5769/318670/15c519a48450/nar00323-0167-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5769/318670/2a6788df8513/nar00323-0167-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5769/318670/620caaf85612/nar00323-0168-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5769/318670/d042594039ba/nar00323-0169-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5769/318670/0db2f4d07d9f/nar00323-0170-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5769/318670/65879c81a334/nar00323-0171-a.jpg

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本文引用的文献

1
Attempts to Detect Agrobacterium tumefaciens DNA in Crown-Gall Tumor Tissue.检测冠瘿瘤组织中根癌土壤杆菌DNA的尝试。
Plant Physiol. 1976 Jul;58(1):100-6. doi: 10.1104/pp.58.1.100.
2
Cytokinin/auxin balance in crown gall tumors is regulated by specific loci in the T-DNA.冠瘿瘤中的细胞分裂素/生长素平衡受 T-DNA 中特定基因座的调节。
Proc Natl Acad Sci U S A. 1983 Jan;80(2):407-11. doi: 10.1073/pnas.80.2.407.
3
Octopine synthase mRNA isolated from sunflower crown gall callus is homologous to the Ti plasmid of Agrobacterium tumefaciens.
从根癌农杆菌 Ti 质粒中分离出一个双植物启动子片段。
EMBO J. 1984 Dec 1;3(12):2723-30. doi: 10.1002/j.1460-2075.1984.tb02202.x.
4
Sequence and functional analysis of the left-hand part of the T-region from the nopaline-type Ti plasmid, pTiC58.胭脂碱型Ti质粒pTiC58 T区左侧部分的序列与功能分析
Plant Mol Biol. 1999 Dec;41(6):765-76. doi: 10.1023/a:1006370207379.
5
Nuclear protein 780BP from cauliflower binds an element in the 780 gene promoter of T-DNA.来自花椰菜的核蛋白780BP与T-DNA的780基因启动子中的一个元件结合。
Plant Mol Biol. 1994 Oct;26(1):377-92. doi: 10.1007/BF00039547.
6
Site-directed mutagenesis of the enhancer region of the 780 gene promoter of T-DNA.T-DNA的780基因启动子增强子区域的定点诱变
Plant Mol Biol. 1995 Oct;29(1):99-108. doi: 10.1007/BF00019122.
7
Selection-expression plasmid vectors for use in genetic transformation of higher plants.用于高等植物遗传转化的选择表达质粒载体。
Nucleic Acids Res. 1985 Oct 11;13(19):6981-98. doi: 10.1093/nar/13.19.6981.
8
Relative strengths of the 35S cauliflower mosaic virus, 1', 2', and nopaline synthase promoters in transformed tobacco sugarbeet and oilseed rape callus tissue.35S花椰菜花叶病毒、胭脂碱合成酶启动子1'、2'以及胭脂碱合成酶启动子在转化的烟草、甜菜和油菜愈伤组织中的相对强度
Mol Gen Genet. 1988 Apr;212(1):182-90. doi: 10.1007/BF00322463.
9
Deletion analysis of the mannopine synthase gene promoter in sunflower crown gall tumors and Agrobacterium tumefaciens.向日葵冠瘿瘤和根癌土壤杆菌中甘露碱合成酶基因启动子的缺失分析
Mol Gen Genet. 1987 May;207(2-3):233-41. doi: 10.1007/BF00331583.
10
Functional domains of a T-DNA promoter active in crown gall tumors.在冠瘿瘤中具有活性的T-DNA启动子的功能结构域。
Mol Cell Biol. 1987 Jan;7(1):59-67. doi: 10.1128/mcb.7.1.59-67.1987.
从向日葵冠瘿组织中分离出的瓜氨酸合酶 mRNA 与根癌农杆菌 Ti 质粒同源。
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4
T-DNA from Agrobacterium Ti plasmid is in the nuclear DNA fraction of crown gall tumor cells.农杆菌 Ti 质粒的 T-DNA 存在于冠瘿瘤细胞的核 DNA 中。
Proc Natl Acad Sci U S A. 1980 Jul;77(7):4060-4. doi: 10.1073/pnas.77.7.4060.
5
Agropine in "null-type" crown gall tumors: Evidence for generality of the opine concept.“无类型”冠瘿瘤中的农杆碱：阿朴碱概念通用性的证据。
Proc Natl Acad Sci U S A. 1980 May;77(5):2693-7. doi: 10.1073/pnas.77.5.2693.
6
Proteins encoded by Agrobacterium tumefaciens Ti plasmid DNA (T-DNA) in crown gall tumors.根癌农杆菌 Ti 质粒 DNA（T-DNA）在冠瘿瘤中编码的蛋白质。
Proc Natl Acad Sci U S A. 1980 May;77(5):2666-70. doi: 10.1073/pnas.77.5.2666.
7
Genetic Identification of functions of TL-DNA transcripts in octopine crown galls.在毛叶冠瘿碱型根瘤中鉴定 TL-DNA 转录本的功能
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8
The TL-DNA in octopine crown-gall tumours codes for seven well-defined polyadenylated transcripts.在毛叶番荔枝冠瘿瘤的 TL-DNA 中，编码了 7 个明确的多聚腺苷酸化转录本。
EMBO J. 1982;1(1):139-46. doi: 10.1002/j.1460-2075.1982.tb01137.x.
9
Identification of sequences involved in the polyadenylation of higher plant nuclear transcripts using Agrobacterium T-DNA genes as models.以农杆菌T-DNA基因作为模型鉴定高等植物核转录本聚腺苷酸化所涉及的序列。
EMBO J. 1983;2(3):419-26. doi: 10.1002/j.1460-2075.1983.tb01439.x.
10
The opine synthase genes carried by Ti plasmids contain all signals necessary for expression in plants.Ti质粒携带的冠瘿碱合成酶基因包含在植物中表达所需的所有信号。
EMBO J. 1983;2(9):1597-603. doi: 10.1002/j.1460-2075.1983.tb01630.x.