Winter J A, Wright R L, Gurley W B
Nucleic Acids Res. 1984 Mar 12;12(5):2391-406. doi: 10.1093/nar/12.5.2391.
Polyadenylated RNA from two octopine type tumor lines (E1, PSCG-15955) was analyzed by RNA blot hybridization and shown to contain five major transcripts homologous to TR DNA. In tobacco E1 tissue, the molecular weights of the TR homologous RNAs are 1.65 kb, 1.55 kb, 1.45 kb, 1.05 kb, and 0.78 kb. The 5' - and 3'-termini of each of the five E1 tumor transcripts were determined by S1 nuclease hybrid protection mapping. The polarity of transcription for the 0.78, 1.05 and 1.65 kb RNAs is from left to right, while the 1.55 and 1.45 kb RNAs are transcribed from right to left. Although we see the same size transcripts in sunflower PSCG-15955 tissue (with the exception of a 0.89 kb RNA in place of the 1.55 kb transcript of E1) there is an apparent difference in the relative abundance of the various RNAs between the two tumor lines.
通过RNA印迹杂交分析了来自两个章鱼碱型肿瘤系（E1、PSCG - 15955）的聚腺苷酸化RNA，结果显示其含有五种与TR DNA同源的主要转录本。在烟草E1组织中，TR同源RNA的分子量分别为1.65 kb、1.55 kb、1.45 kb、1.05 kb和0.78 kb。通过S1核酸酶杂交保护图谱确定了E1肿瘤的这五种转录本各自的5'端和3'端。0.78 kb、1.05 kb和1.65 kb RNA的转录极性是从左到右，而1.55 kb和1.45 kb RNA是从右到左转录。尽管我们在向日葵PSCG - 15955组织中看到了相同大小的转录本（除了用0.89 kb RNA取代了E1的1.55 kb转录本），但这两个肿瘤系中各种RNA的相对丰度存在明显差异。
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