De Simone C, Cilli A, Zanzoglu S, Vullo V, Delia S, Sorice F
Trans R Soc Trop Med Hyg. 1984;78(1):64-8. doi: 10.1016/0035-9203(84)90175-5.
Sera from 15 patients with Entamoeba histolytica infection were tested for anti T-cell antibodies by assessing cross-reacting specificities with the antigens defined by an anti-Ia hybridoma antibody. T cells prepared by sheep erythrocyte rosetting were preincubated with the test sera and then with the anti-Ia antibody. Binding of the specific monoclonal antibody was assessed by rosetting with ox erythrocytes conjugated with goat anti-mouse IgG. Eight sera from amoebic patients were found to block the binding of monoclonal mouse hybridoma anti Ia-antibody to T cells. Blocking of anti-Ia binding was not due to Fc IgG receptor binding by immune complexes nor was it HLA-DR restricted. T cells pre-treatment with the amoebic sera positive for anti-Ia activity showed reduced activity when tested in the autologous mixed lymphocyte reaction (AMLR). The results of our study seem to suggest the existence of specific anomalies of immunoregulation during E. histolytica infection which may play a role in inducing immune disregulation in vivo.
通过评估与抗Ia杂交瘤抗体所定义抗原的交叉反应特异性，对15例溶组织内阿米巴感染患者的血清进行了抗T细胞抗体检测。通过绵羊红细胞花环法制备的T细胞先与检测血清预孵育，然后再与抗Ia抗体孵育。通过与结合山羊抗小鼠IgG的牛红细胞进行花环试验来评估特异性单克隆抗体的结合情况。发现8例阿米巴病患者的血清可阻断单克隆小鼠杂交瘤抗Ia抗体与T细胞的结合。抗Ia结合的阻断并非由于免疫复合物与Fc IgG受体结合所致，也不受HLA-DR限制。用抗Ia活性呈阳性的阿米巴血清预处理后的T细胞，在自体混合淋巴细胞反应（AMLR）中检测时活性降低。我们的研究结果似乎表明，在溶组织内阿米巴感染期间存在特异性免疫调节异常，这可能在体内诱导免疫失调中起作用。
