Okudaira K, Searles R P, Ceuppens J L, Goodwin J S, Williams R C
J Clin Invest. 1982 Jan;69(1):17-24. doi: 10.1172/jci110428.
Antileukocyte antibodies in sera from patients with systemic lupus erythematosus (SLE) were characterized by determining cross-reacting specificies with the antigens defined by OKT3, OKT4, OKT8, OKM1 and anti-Ia hybridoma antibodies (Abs). T cells were prepared by sheep erythrocyte (E) rosetting after removal of adherent cells. T cells, or non-T cells, were preincubated with SLE sera at 4 degrees C and then with monoclonal Abs. Binding by specific monoclonal Abs was assessed by two methods: rosetting with ox erythrocytes conjugated with goat anti-mouse IgG and also in the fluorescence-activated cell sorter using fluorescein isothiocyanate-conjugated goat anti-mouse IgG. Using the rosetting method, we found that sera from SLE can block the binding of monoclonal mouse hybridoma anti-Ia Abs to T cells; the blocking of other monoclonal Abs was not consistent. Using fluorescence-activated cell sorter analysis, preincubation with SLE sera lowered the intensity of staining and total percentage of either T or non-T cells stained by monoclonal anti-Ia Abs. Blocking of anti-Ia Abs binding by SLE sera was not histocompatibility leukocyte antigen (HLA)-DR restricted and was not due to Fc receptor binding. These results indicated that antibodies in SLE sera react with structures contiguous to or identical with Ia determinants. Anti-Ia activities in SLE sera correlate with SLE disease activity. In addition, there was a significant negative correlation between anti-Ia blocking activity in the sera and the percentage of Ia-positive T cells in the blood of SLE patients. Antibodies in SLE sera with anti-Ia blocking activity may play an important role in immune dysregulation in SLE patients.
通过确定系统性红斑狼疮（SLE）患者血清中的抗白细胞抗体与由OKT3、OKT4、OKT8、OKM1和抗Ia杂交瘤抗体（Abs）所定义抗原的交叉反应特异性，对这些抗体进行了特性分析。去除贴壁细胞后，通过绵羊红细胞（E）花环形成法制备T细胞。将T细胞或非T细胞在4℃下与SLE血清预孵育，然后与单克隆抗体孵育。通过两种方法评估特异性单克隆抗体的结合：与结合了山羊抗小鼠IgG的牛红细胞进行花环形成，以及在荧光激活细胞分选仪中使用异硫氰酸荧光素结合的山羊抗小鼠IgG。使用花环形成法，我们发现SLE患者的血清可阻断单克隆小鼠杂交瘤抗Ia抗体与T细胞的结合；对其他单克隆抗体的阻断则不一致。使用荧光激活细胞分选仪分析，与SLE血清预孵育会降低单克隆抗Ia抗体染色的T细胞或非T细胞的染色强度和总百分比。SLE血清对抗Ia抗体结合的阻断不受组织相容性白细胞抗原（HLA）-DR限制，也不是由于Fc受体结合。这些结果表明，SLE血清中的抗体与Ia决定簇相邻或相同的结构发生反应。SLE血清中的抗Ia活性与SLE疾病活动相关。此外，血清中的抗Ia阻断活性与SLE患者血液中Ia阳性T细胞的百分比之间存在显著负相关。具有抗Ia阻断活性的SLE血清中的抗体可能在SLE患者的免疫失调中起重要作用。
