Sonnenfeld G, Hudgens R W, Streips U N
J Interferon Res. 1984;4(1):129-33. doi: 10.1089/jir.1984.4.129.
Several carcinogens have been shown to depress in vitro interferon (IFN) induction, while closely matched weakly or noncarcinogenic analogues had no effect on IFN induction. In this study, specific carcinogens that were difficult to detect by the Ames Salmonella assay were tested for their effects of IFN-alpha/beta induction. The carcinogens were applied to mouse embryo fibroblast cultures, and were then removed. IFN-alpha/beta induction was then carried out using polyriboinosinic-polyribocytidylic acid. Application of urethane, which was undetectable by the Salmonella assay except under special conditions, significantly depressed IFN-alpha/beta induction. Treatment with cyclophosphamide or dimethylcarbomyl chloride, both of which required extremely large dosages to be detected by the Salmonella assay, had no effect on IFN-alpha/beta induction at the dosages used. Higher dosages of these carcinogens were toxic to the cells used in this system.
已有研究表明，几种致癌物可在体外抑制干扰素（IFN）的诱导，而结构紧密匹配的弱致癌物或非致癌物类似物对IFN诱导没有影响。在本研究中，对难以通过艾姆斯沙门氏菌试验检测到的特定致癌物进行了IFN-α/β诱导作用测试。将这些致癌物应用于小鼠胚胎成纤维细胞培养物，然后去除。随后使用聚肌苷酸-聚胞苷酸进行IFN-α/β诱导。除特殊条件下外，沙门氏菌试验无法检测到的氨基甲酸乙酯的应用显著抑制了IFN-α/β诱导。环磷酰胺或二甲基氨基甲酰氯在沙门氏菌试验中需要极高剂量才能检测到，在所使用的剂量下，它们对IFN-α/β诱导没有影响。这些致癌物的较高剂量对本系统中使用的细胞有毒性。
